EurekaMag.com logo
+ Site Statistics
References:
48,252,460
Abstracts:
22,798,453
+ Resolve Accession
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
Submit PDF Full TextSubmit PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Differences in thromboxane production between neonatal and adult platelets in response to arachidonic acid and epinephrine


, : Differences in thromboxane production between neonatal and adult platelets in response to arachidonic acid and epinephrine. Pediatric Research 18(9): 823-826

The possible role of thromboxane in the impaired function of neonatal platelets was studied. In platelet-rich plasma thromboxane production (measured by radioimmunoassay of thromboxane B2 [TXB2]) was not different between neonates and adults when stimulated by thrombin (at 0.1 or 1.0 U/ml) or collagen (70 .mu.g/ml) although neonatal platelets produced decreased TXB2 postepinephrine stimulation. In response to 1 U/ml thrombin, adult and neonatal platelet-rich plasmas produced mean values of 3.41 .+-. 0.35 (SEM) and 3.11 .+-. 0.49 pmol of TXB2/106 platelets, respectively. Production of TXB2 in response to 0.1 U/ml thrombin was not dissimilar between neonates and adults. When collagen was used as the aggregating agnet, TXB2 production was also not significantly different. In response to 200 .mu.M epinephrine, adult platelets produced 1.03 .+-. 0.39 pmol TXB2/106 platelets while neonatal platelet TXB2 production was significantly decreased (0.15 .+-. 0.04). TXB2 production in response to AA, however, was markedly elevated in neonatal platelet-rich plasma. When 200 and 400 .mu.M concentrations of AA were used as the aggregating stimuli, neonatal platelet rich plasma produced 3.17 .+-. 0.77 and 8.0 .+-. 1.47 pmol TXB2/106 platelets, respectively. These values were significantly elevated when compared to mean values of 0.41 .+-. 0.10 and 3.32 .+-. 0.15 pmol in adult platelet-rich plasma. This elevated TXB2 production was not, however, inherent in neonatal platelets since when washed platelets were studied results were reversed. Adult platelets produced more TXB2 at all doses of AA evaluated. The elevated response to exogenous AA observed in neonatal platelet-rich plasma apparently results from as yet undetermined plasma factors. The reported deficiencies in platelet function in the newborn clearly do not result from deficient TXB2 production poststimulation with the physiologic aggregating agents collagen and thrombin.

(PDF same-day service: $19.90)

Order PDF Full Text

Close
Close

Click here to order any other PDF Full Text


Accession: 005151679

PMID: 6435080

DOI: 10.1203/00006450-198409000-00003

PDF Full Text: Submit


Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:
Close
Close

Other references

Martensz N.D.; Scaramuzzi R.J.; Van Look P.F.A., 1979: Plasma concentrations of luteinizing hormone and follicle stimulating hormone during anestrus in ewes actively immunized against estradiol 17 beta estrone or testosterone. The concentrations of LH [luteinizing hormone] and FSH [follicle stimulating hormone] in jugular venous plasma were measured during anestrus in control ewes and ewes actively immunized against 17.beta.-estradiol-6-(O-carboxymethyl)oxime-bovine ser...

Gizatullina S.S.; Birger M.O.; Kulinich L.I.; Fish N.F.; Mazitova O.P.; Biryukova N.V., 1991: Method for assessing the status of human intestinal microflora according to the number of adhesive active bacteria and the type of adhesins. The method for the evaluation of the state of intestinal microflora in the diagnosis of enteric diseases by the determination of the number of actively adhesive bacteria and the type of adhesins in the primary culture of human microflora has been...

Bennforf K.; Friedrich M.; Hirche H., 1991: Reoxygenation induced arrhythmogenic transient inward currents in isolated cells of the guinea pig heart. Transient inward currents (Iti), activated by a rise in intracellular Ca concentration, are believed to trigger cardiac arrhythmias in reperfused hearts. In this report Iti in isolated cardiocytes from the guinea-pig were evoked by reoxygenation f...

Bello, A.K.; Hemmelgarn, B.; Lin, M.; Manns, B.; Klarenbach, S.; Thompson, S.; James, M.; Tonelli, M., 2013: Impact of remote location on quality care delivery and relationships to adverse health outcomes in patients with diabetes and chronic kidney disease. To investigate the relation of residence location, markers of good quality healthcare and adverse clinical outcomes in patients with diabetes and chronic kidney disease (CKD). We identified 31 337 individuals with diabetes and estimated glomerular...

Jacobson, Ga; Aldridge, Dj; Kirkpatrick, Ja, 1976: The stability of fats and oils in foods. Objective Methods for Food Evaluation Proceedings of a Symposium: ub 1976) 185-203

Anthony H.M.; Madsen K.E.; Mason M.K.; Templeman G.H., 1981: Lung cancer immune status histo pathology and smoking is oat cell carcinoma lympho dependent. Life table analysis and partial correlation analysis were used to examine the importance and interaction of those factors reported to affect prognosis in lung cancer in a group of men taking part in a recent trial. In patients with squamous carcin...

Sato, Tooru; Oikawa, Keishi; Miyazaki, Sakaki, 1962: On the regeneration methods and effect of fertilization on Akamatsu. Twelve types of regeneration tests were carried out to test the production of P. densiflora under short-term rotation. Artificial direct seeding was considered to be the most efficient method. Fertilized stands were superior in quality of yield at...

Loos, M. A.; Louw, H. A., 1965: The influence of calcium carbonate amendments on the nodulation of white clover in the acid soils of George. The influence of calcium carbonate amendments on the nodulation of white clover in an acid soil from George has been investigated. The pH (4.8) of the un-amended soil was not too low to permit the survival and multiplication of the clover rhizobia...

Ferreira A.S.; Casela C.R., 1986: Pathogenic races of colletotrichum graminicola causal agent of anthracnose in sorghum sorghum bicolor. This work was carried out to determine the occurrence of pathogenic races of Colletotrichum graminicola, causal agent of anthracnose in sorghum, through the utilization of a set of twelve differential cultivars. Seven single spores isolates of the...

Vrezec, Al E-mail: al.vrezecnib.si., 2006: Is the grey partridge Perdix perdix population decline in Slovenia the result of apparent competition with the pheasant Phasianus colchicus? Ali je vzrok upada populacije jerebice Perdix perdix v Sloveniji prikrita kompeticija s fazanom Phasianus colchicus?. Changes in agriculture are believed to be the main reason for the drastic decline in the Grey Partridge Perdix perdix population in Europe since 1950. Tompkins et al. (1999) suggested that apparent competition with the Pheasant Phasianus colchicus...