EurekaMag.com logo
+ Site Statistics
References:
53,214,146
Abstracts:
29,074,682
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Differences in volumes of sister nuclei in bi nucleate cells evidence for asymmetry of mitosis






Canadian Journal of Botany 56(19): 2362-2369

Differences in volumes of sister nuclei in bi nucleate cells evidence for asymmetry of mitosis

Roots of Zea mays cv. Golden Bantam were treated with a 0.1% solution of caffeine for 1-4 h and allowed to recover for up to 17 h. Binucleate cells were present in the root cap and in the meristem. Nuclear volumes were determined for the pairs of sister nuclei in the binucleate cells. In row I cells of the root cap, 41% of pairs of sister nuclei had different volumes in roots treated with caffeine for 1-4 h or for 2 h and then allowed to recover for 5 h. In the period 2 + 6 to 2 + 17 h, however, 70% of the pairs differed in volume. Volumes of sister nuclei present in the same cell differ soon after they enter G1. They also differ in the period 2 + 6 to 2 + 17 h when mean nuclear volume increases from 145-243 .mu.m3. Both aspects suggest that sister nuclei differ in their ability to grow. In most binucleate cells, the larger nucleus was in the position it would have occupied if it lay in a row I cell. A gradient apparently exists, in a row I cell, of some factor that regulates nuclear growth. The consequence of this gradient is that sister nuclei grow differentially even though they share a common cytoplasm. Mitosis in a row I cell appears to be asymmetrical. Cells induced to become binucleate were inhibited by the caffeine treatment. Only 9% of the binucleate cells completed a cell cycle and divided and, for some of them, cycle time was increased.

(PDF 0-2 workdays service: $29.90)

Accession: 005151745



Related references

Differential behavior of sister nuclei in methyl xanthine induced bi nucleate cells. Experimental Cell Research 116(2): 450-454, 1978

Interphase development and beginning of mitosis in the different nuclei of poly nucleate homokaryotic cells. Chromosoma (Berlin) 36(1): 100-111, 1971

Differences in protein content of sister nuclei: evidence from binucleate and mononucleate cells. Canadian Journal of Biochemistry 60(3): 371-378, 1982

Further studies on mitosis of bi nucleate and multi nucleate neopl hela cells human. Scandinavian Journal of Clinical & Laboratory Investigation Supplement 22(106): 79-96, 1968

Early phenotypic asymmetry of sister oligodendrocyte progenitor cells after mitosis and its modulation by aging and extrinsic factors. Glia 63(2): 271-286, 2015

Sequence of transition to mitosis in wheat root sister cells and their differences in the duration of mitotic cycles. Botanicheskii Zhurnal (St Petersburg) 60(2): 188-198, 1975

Initiation of dna synthesis and mitosis in multi nucleate hela cells obtained by fusing cells from different phases of the cell cycle. Journal of Cell Biology 43(2 PART 2): 110A-111A, 1969

Synchronous dna synthesis and mitosis in multi nucleate cells with 1 chromosome in each nucleus. Chromosoma (Berlin) 89(3): 197-200, 1984

Mammalian cell fusion part 6 regulation of mitosis in bi nucleate hela cells. Experimental Cell Research 90(1): 40-46, 1974

The role of protein synthesis in cell progression through g 2 and mitosis in plant multi nucleate cells. Biology of the Cell (Paris) 46(2): 161-174, 1982