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Differences of antigen composition of rheumatoid arthritic and nonrheumatoid arthritic derived tissue cultured synovial cells as detected by monkey anti rheumatoid arthritic synovial cell sera

, : Differences of antigen composition of rheumatoid arthritic and nonrheumatoid arthritic derived tissue cultured synovial cells as detected by monkey anti rheumatoid arthritic synovial cell sera. Journal of Rheumatology 8(2): 204-213

Antisera produced in a rhesus monkey by injections of RA-derived synovial cells grown in tissue culture react preferentially with synovial cells compared to fibroblastic cell strains of other tissues. The antisera also detected differences of the antigenic components of RA and non-RA synovial cells utilizing a number of immunochemical techniques. Following adsorptions with cross-reactive antigen sources the antisera exhibited quantitative differences of reactivity to either RA or non-RA cells by immunofluorescence and complement-mediated cytotoxicity assays. Fetal calf serum antigens differentially adhere to RA and non-RA synovial cells. Antigens in synovial fluid cross-reactive with synovial cell antigens are expressed to a greater degree on RA-derived synovial cells. The results substantiate other reports that synovial cells from RA individuals are different from non-RA cells by a number of growth, metabolic and antigenic characteristics.

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