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Different arrangements of phago lysosome membranes which depend upon the particles phagocytized observation with markers of the 2 sides of plasma membranes


, : Different arrangements of phago lysosome membranes which depend upon the particles phagocytized observation with markers of the 2 sides of plasma membranes. Experimental Cell Research 111(2): 437-449

Phagolysosome vesicles containing either polystyrene latex (PLP), or PLP plus bovine serum albumin (PLP-BSA), or paraffin oil emulsified with BSA (POE-BSA) were prepared from guinea pig polymorphonuclear leukocytes (gp-PMNL). They were compared using the specific markers of the 2 sides of the plasma membrane of gp-PMNL characterized previously. Anticell surface Ig[immunoglobulin]G (Anti-C-S IgG) which binds exclusively to the outer side of plasma membranes was bond to the cytoplasmic side of all 3 types of phagolysosomes. Anti-100,000 g sup IgG which binds exclusively to the cytoplasmic side of plasma membranes of gp-PMNL did not bind to the cytoplasmic side of these phagolysosomes, as shown by immunoferritin technique. Influenza virus, which is a marker of the outer surface of plasma membranes bound to the cytoplasmic side of POE-BSA phagolysosomes but not PLP or PLP-BSA phagolysosomes, as demonstrated by EM. The content of myosin, another marker of the cytoplasmic side of plasma membranes differed in these phagolysosomes, as shown by sodium dodecyl sulfate polyacrylamide gel electrophoresis: it was very low in PLP- and POE-BSA phagolysosomes but high in PLP-BSA phagolysosomes. Myosin was found on the cytoplasmic side of PLP-BSA phagolysosomes by the improved immunoferritin technique reported previously. These phagolysosomes, containing different particles and having different arragements of membrane components, showed different efficiencies of fusion with lysosomes. The possible mechanism of the specific fusion of phagosomes with lysosomes was discussed in relation to these different features of phagolysosomal membranes.

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