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Different asparagine linked sugar chains on the 2 poly peptide chains of human chorionic gonadotropin


, : Different asparagine linked sugar chains on the 2 poly peptide chains of human chorionic gonadotropin. Biochemical & Biophysical Research Communications 97(2): 772-778

Human chorionic gonadotropin (hCG) purified from placenta, like urinary hCG, has the sialylated forms of 3 neutral oligosaccharides: Gal.beta.1 .fwdarw. 4GlcNAc.beta.1 .fwdarw. 2Man.alpha.1 .fwdarw. 6 (Gal.beta.1 .fwdarw. 4GlcNAc.beta.1 .fwdarw. 2 Man.alpha.1 .fwdarw. 3) Man.beta.1 .fwdarw. 4GlcNAc.beta.1 .fwdarw. 4 (Fuc.alpha.1 .fwdarw. 6) GlcNAc (N-1), Gal.beta.1 .fwdarw. 4GlcNAc.beta.1 .fwdarw. 2Man.alpha.1 .fwdarw. 6 (Gal.beta.1 .fwdarw. 4GlcNAc.beta.1 .fwdarw. 2Man.alpha.1 .fwdarw. 3) Man.beta.1 .fwdarw. 4GlcNAc.beta.1 .fwdarw. 4GlcNAc (N-2) and Man.alpha.1 .fwdarw. 6 (Gal.beta.1 .fwdarw. 4GlcNAc.beta.1 .fwdarw. 2Man.alpha.1 .fwdarw. 3) Man.beta.1 .fwdarw. 4 GlcNAc.beta.1 .fwdarw. 4GlcNAc (N-3). Gel permeation chromatographic analysis of oligosaccharides released from .alpha.- and .beta.-subunits of placental hCG revealed that the .alpha.-subunit has 1 each of sialylated N-2 and N-3, while the .beta.-subunit has 1 each of sialylated N-1 and N -2.

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Related references

Mizuochi, T.; Nishimura, R.; Derappe, C.; Taniguchi, T.; Hamamoto, T.; Mochizuki, M.; Kobata, A., 1983: Structures of the asparagine-linked sugar chains of human chorionic gonadotropin produced in choriocarcinoma. Appearance of triantennary sugar chains and unique biantennary sugar chains. Human chorionic gonadotropin (hCG) highly purified from urine of the patient with choriocarcinoma contains four asparagine-linked sugar chains in one molecule. The sugar chains were quantitatively liberated as radioactive oligosaccharides from pol...

Mizuochi, T.; Kobata, A., 1980: Different asparagine-linked sugar chains on the two polypeptide chains of human chorionic gonadotropin. Biochemical and Biophysical Research Communications 97(2): 772-778

Endo, Y.; Yamashita, K.; Tachibana, Y.; Tojo, S.; Kobata, A., 1979: Structures of the asparagine-linked sugar chains of human chorionic gonadotropin. The asparagine-linked sugar chains of human chorionic gonadotropin were released from the polypeptide moiety by hydrazinolysis followed by N-acetylation and NaB3H4 reduction. More than 90% of the released radioactive oligosaccharides contained N-a...

Kobata A.; Mizuochi T., 1984: Structures and functions of the asparagine linked sugar chains of human chorionic gonadotropin. Gunma Symposia on Endocrinology 21: 139-150

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