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Different base base mismatches are corrected with different efficiencies by the methyl directed dna mismatch repair system of escherichia coli



Different base base mismatches are corrected with different efficiencies by the methyl directed dna mismatch repair system of escherichia coli



Cell 38(3): 879-888



The efficiency of methyl-directed DNA mismatch-repair of E. coli acting in vivo on heteroduplex genomes of phage M13 was strongly dependent on the nature of the base/base mismatch to be corrected. Three efficiency classes were characterized: high (T/G, C/A and G/G); intermeditae (A/A); and low (G/A, A/G, T/T, C/C, C/T and T/C). Methyl-directed DNA mismatch repair was lost completely for any type of mismatch in strains carrying mutL or mutS mutations. Data obtained with a mutH mutant suggest that this locus is involved in methyl-dependent DNA strand discrimination. A functional correlation is suggested between the differential repair efficiencies and the frequencies of the corresponding replication errors.

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Accession: 005151897

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