+ Site Statistics
+ Resolve Accession
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
Submit PDF Full TextSubmit PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Different baseline sister chromatid exchange levels in density fractionated human lymphocytes

, : Different baseline sister chromatid exchange levels in density fractionated human lymphocytes. Human Genetics 73(2): 114-118

Different activation states of B and T lymphocytes, as manifested by differences in cell density, were obtained by Percoll density centrifugation of unstimulated human lymphocytes. Four different density fractions were defined: B cells with low (1.043 g/ml) and high (1.056) density, and T cells with low (1.067) and high (1.077) density, respectively. Sister chromatid exchange (SCE) conditions and proliferation rates were determined. Total B cells, stimulated by the bacterial mitogen Branhamella, had 4.6 SCE per cell, the lowest mean baseline SCE level recorded among lymphocytes. The growth rate was intermediate between that of low and high density T cells. The two T cell fractions stimulated by phytohemagglutinin (PHA) had different baseline SCE frequencies and different growth characteristics: the low density cells had 5.7 SCEs per cell and a short cell cycle, whereas high density cells had 12.5 SCEs per cell and a longer cell cycle. The differences in baseline SCE frequency and growth characteristics between the two T cell fractions seem to be correlated with the differences in the activation state as reflected by the cell density. Both high and low density T cells are G0 populations which supposedly differ with respect to previous history in vivo such as age and contact with antigens. The reason why these cells react differently to bromodeoxyuridine (BrdU) is unknown, but differences in intracellular DNA precursor pools and enzyme activities might play a role.

Order PDF Full Text


Click here to order any other PDF Full Text

Accession: 005151899

PMID: 3721498

DOI: 10.1007/bf00291598

PDF Full Text: Different baseline sister chromatid exchange levels in density fractionated human lymphocytes

Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:

Other references

Matsuo, Yoshinori, 2000: Effect of genome-wide factor on the codon usage of genes in Drosophila. Genes & Genetic Systems 75(6): 402, December

Nakajima K.; Bunko H.; Maeda T.; Yamada M.; Hisada K., 1981: Evaluation of left ventricular wall motion with functional images. Kaku Igaku 18(5): 726

Benardini, J.N.; Venkateswaran, K., 2016: Application of the ATP assay to rapidly assess cleanliness of spacecraft surfaces: a path to set a standard for future missions. NlmCategory="UNASSIGNED">The National Aeronautics and Space Administration (NASA) measures and validates the biological cleanliness of spacecraft surfaces by counting endospores using the NASA standard assay (NSA). NASA has also appr...

Aziz, O.; Inam, A.; Trannum, K., 1993: Effect of treated refinery waste water on nitrate reductase activity of Vigna radiata L. The study was carried out on Vigna radiata var. T-44 and K-8512 to study the effect of treated refinery effluent on nitrate reductase activity (NRA). The enzyme activity was stimulated more by effluent application in var. T-44 as compared to var....

Handler, M.Z.; Hamilton, H.; Aires, D., 2012: Treatment of peristomal pyoderma gangrenosum with topical crushed dapsone. A 27-year-old male with a history of Crohn's disease was treated for chronic pyoderma gangrenosum at his stoma site. Treatment with topical application of crushed dapsone resulted in improvement of his pyoderma gangrenosum. Crushed dapsone ma...

Ye, H.J., 1985: Sensitivity of botulinum toxins on mice, rabbits and monkeys. Zhonghua Yu Fang Yi Xue Za Zhi 19(5): 261-262

Jia, S.; Fleischer, J.W., 2010: Nonlinear light propagation in fractal waveguide arrays. We experimentally and numerically study nonlinear light propagation in a fractal waveguide array. We consider a nested set of periodic arrays and examine energy transport as a function of band structure, nonlinearity, and probe beam geometry. Expe...

Ruiz Feria, C.A.; Yang, Y.; Nishimura, H., 2002: Arginine reduces aortic lesions without reducing blood pressure in young white Leghorn chicks. Poultry Science 81(Supplement 1): 108-109

Yik, J.H.N.; Hu, Z'ang.; Kumari, R.; Christiansen, B.A.; Haudenschild, D.R., 2014: Cyclin-dependent kinase 9 inhibition protects cartilage from the catabolic effects of proinflammatory cytokines. Cyclin-dependent kinase 9 (CDK-9) controls the activation of primary inflammatory response genes. The purpose of this study was to determine whether CDK-9 inhibition protects cartilage from the catabolic effects of proinflammatory cytokines. Human...

Grahn, P.J.; Vaishya, S.; Knight, A.M.; Chen, B.K.; Schmeichel, A.M.; Currier, B.L.; Spinner, R.J.; Yaszemski, M.J.; Windebank, A.J., 2015: Implantation of cauda equina nerve roots through a biodegradable scaffold at the conus medullaris in rat. Traumatic injuries occurring at the conus medullaris of the spinal cord cause permanent damage both to the central nervous system and to the cauda equina nerve roots. This proof-of-concept study was to determine whether implanting the nerve roots...