+ Translate

Different biochemical properties of fetal and adult red cell hexo kinase ec iso enzymes

, : Different biochemical properties of fetal and adult red cell hexo kinase ec iso enzymes. Hoppe Seyler's Zeitschrift fuer Physiologische Chemie 361(6): 829-838

The distribution of hexokinase isoenzymes was analyzed in erythrocytes from adults and newborn infants. Isolation by DEAE-cellulose chromatography revealed 1 main peak (Ia) in erythrocytes from adults similar to hexokinase isoenzyme I from rat liver and a smaller peak (Ib) which was eluted at a lower conductivity than rat liver isoenzyme II. In erythrocytes from newborn infants the peak Ib dominated, isoenzyme Ia was the smaller one. In adult erythrocytes an additional isoenzyme was detected which resembled rat liver isoenzyme III; this isoenzyme was absent in erythrocytes from newborn infants. The erythrocytic isoenzymes Ia and Ib differed in their kinetic properties. Isoenzyme Ia had a slightly higher affinity or glucose (Km 47 .mu.M) than isoenzyme Ib (Km 100 .mu.M). In the presence of 5 mM Pi isoenzyme Ib was more inhibited by glucose 6-phosphate than isoenzyme Ia. 2,3-Diphosphoglycerate inhibited isoenzyme Ib more than isoenzyme Ia.

(PDF 0-2 workdays service)

Accession: 005151939

Submit PDF Full Text: Here

Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:

Related references

Anonymous, 1975: Hexo kinase isozymes in human neoplastic and fetal tissues the existence of hexo kinase ii ec 2711 in malignant tumors and in placenta

Schroeter W., 1979: Fetal hexo kinase iso enzymes and hemo globin f in children with hematologic diseases. Pediatric Research: 956

Faulkner, A.; Jones, C.T., 1976: Hexo kinase ec iso enzymes ec in tissues of the adult and developing guinea pig. Changes in the activities and isoenzyme distribution of hexokinase were determined in a number of tissues during the development of the guinea pig. The total activity in the fetal liver showed a large fall during the 2nd half of gestation to reach...

Monakhov, N.K.; Shvartsman, A.L.; Neifakh, S.A., 1975: Physicochemical and kinetic properties of hexo kinase iso enzymes from normal and tumor human tissues. Individual hexokinase isoenzymes (isoHK) are isolated from normal and malignant human stomach mucosa. IsoHK from tumor tissue had a KM for glucose 10 times as low as isoHK from normal tissue. Molecular weights of individual isoHK from normal and t...

Monakhov N.K.; Shvartsman A.L.; Neifakh S.A., 1975: Physicochemical and kinetic properties of hexo kinase iso enzymes from normal and tumoral human tissue. Biochemistry (Moscow) 40(5 PART 1): (1976)

Kamikashi, T.; Kizaki, H.; Murakami, K.; Ishibashi, S., 1974: Difference in kinetic properties between hexo kinase ec type i iso enzymes from various rat tissues with reference to the effect of a thiol inhibitor. Biochemical Journal 137(1): 139-142

Anonymous, 1973: Cell physiological and biochemical markers of reticulocyte maturation part 3 behavior of hexo kinase ec 2711 and gluco kinase ec 2712 of red blood cells in bleeding anemia of rabbit

Travis S.F.; D.A.varado C.M.; Cannon E.; Delivoria Papadopoulos M., 1977: Interdependence of red cell hexo kinase inorganic phosphorus and 2 3 di phospho glycerate in the developing fetal lamb. Pediatric Research 11(4): 483

Crompton M.; Birt L.M., 1969: Changes in the in vitro activity of glycolytic enzymes during metamorphosis of the blow fly lucilia cuprina enz glycogen phosphorylase enz hexo kinase enz phosphofructo kinase enz aldolase enz glyceraldehyde 3 phosphate dehydrogenase enz 3 phosphoglycerate kinase enz pyruvic kinase. Journal of Insect Physiology 15(7): 1119-1127

Stocchi, V.; Magnani, M.; Novelli, G.; Dacha, M.; Fornaini, G., 1983: Pig red blood cell hexo kinase ec evidence for the presence of hexo kinase type ii and type iii and their purification and characterization. Pig erythrocytes, in contrast to red blood cells from other mammals were shown to contain hexokinase (EC types II and III. Hexokinase type III is the predominant form, accounts for 98% of the total glucose phosphorylating activity, and wa...