EurekaMag.com logo
+ Site Statistics
References:
53,214,146
Abstracts:
29,074,682
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Different biochemical properties of fetal and adult red cell hexo kinase ec 2.7.1.1 iso enzymes


Hoppe Seyler's Zeitschrift fuer Physiologische Chemie 361(6): 829-838
Different biochemical properties of fetal and adult red cell hexo kinase ec 2.7.1.1 iso enzymes
The distribution of hexokinase isoenzymes was analyzed in erythrocytes from adults and newborn infants. Isolation by DEAE-cellulose chromatography revealed 1 main peak (Ia) in erythrocytes from adults similar to hexokinase isoenzyme I from rat liver and a smaller peak (Ib) which was eluted at a lower conductivity than rat liver isoenzyme II. In erythrocytes from newborn infants the peak Ib dominated, isoenzyme Ia was the smaller one. In adult erythrocytes an additional isoenzyme was detected which resembled rat liver isoenzyme III; this isoenzyme was absent in erythrocytes from newborn infants. The erythrocytic isoenzymes Ia and Ib differed in their kinetic properties. Isoenzyme Ia had a slightly higher affinity or glucose (Km 47 .mu.M) than isoenzyme Ib (Km 100 .mu.M). In the presence of 5 mM Pi isoenzyme Ib was more inhibited by glucose 6-phosphate than isoenzyme Ia. 2,3-Diphosphoglycerate inhibited isoenzyme Ib more than isoenzyme Ia.

(PDF 0-2 workdays service: $29.90)

Accession: 005151939



Related references

Hexo kinase isozymes in human neoplastic and fetal tissues the existence of hexo kinase ii ec 2711 in malignant tumors and in placenta. 1975

Fetal hexo kinase iso enzymes and hemo globin f in children with hematologic diseases. Pediatric Research: 956, 1979

Hexo kinase ec 2.7.1.1 iso enzymes ec 2.7.1.2 in tissues of the adult and developing guinea pig. Archives of Biochemistry and Biophysics 175(2): 477-486, 1976

Physicochemical and kinetic properties of hexo kinase iso enzymes from normal and tumor human tissues. Biokhimiya 40(5): 915-922, 1975

Physicochemical and kinetic properties of hexo kinase iso enzymes from normal and tumoral human tissue. Biochemistry (Moscow) 40(5 PART 1): (1976), 1975

Difference in kinetic properties between hexo kinase ec 2.7.1.1 type i iso enzymes from various rat tissues with reference to the effect of a thiol inhibitor. Biochemical Journal 137(1): 139-142, 1974

Cell physiological and biochemical markers of reticulocyte maturation part 3 behavior of hexo kinase ec 2711 and gluco kinase ec 2712 of red blood cells in bleeding anemia of rabbit. 1973

Interdependence of red cell hexo kinase inorganic phosphorus and 2 3 di phospho glycerate in the developing fetal lamb. Pediatric Research 11(4): 483, 1977

Changes in the in vitro activity of glycolytic enzymes during metamorphosis of the blow fly lucilia cuprina enz glycogen phosphorylase enz hexo kinase enz phosphofructo kinase enz aldolase enz glyceraldehyde 3 phosphate dehydrogenase enz 3 phosphoglycerate kinase enz pyruvic kinase. Journal of Insect Physiology 15(7): 1119-1127, 1969

Pig red blood cell hexo kinase ec 2.7.1.1 evidence for the presence of hexo kinase type ii and type iii and their purification and characterization. Archives of Biochemistry and Biophysics 226(1): 365-376, 1983