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Different biosynthetic pathways of the pyrimidine moiety of thiamin in prokaryotes and eukaryotes


, : Different biosynthetic pathways of the pyrimidine moiety of thiamin in prokaryotes and eukaryotes. Biochimica et Biophysica Acta 756(1): 41-48

[14C]Formate is incorporated into the C-2 of the pyrimidine moiety of thiamin by Escherichia coli and Salmonella typhimurium. In Saccharomyces cerevisiae, it is incorporated into C-4. Radioactive carbons of [1-14C]glycine and [2-14C]glycine are incorporated by S. typhimurium into the C-4 and C-6 of the pyrimidine, respectively, but not by S. cerevisiae. Procaryotes and eucaryotes probably have different biosynthetic pathways for pyrimidine. The procaryotes tested incorporated [14C]formate into the C-2 and the eucaryotes incorporated it into the C-4 of the pyrimidine.

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