+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Differential effects of protoporphyrin and uroporphyrin on murine mast cells

Differential effects of protoporphyrin and uroporphyrin on murine mast cells

Journal of Investigative Dermatology 88(3): 281-286

To investigate the mechanisms responsible for the distinct cutaneous manifestations of erythropoietic protoporphyria and porphyria cutanea tarda, the effects of protoporphyrin (PP) and uroporphyrin (URO), the predominant porphyrins in the respective disease, on mast cells were examined. Release of preformed and generated mediators was assessed by the release of radioactivity from cells labeled with [3H]serotonin and [14C]arachidonic acid, respectively. Clinically relevant doses of PP (25-500 ng/ml) and 396-407 nm irradiation (3-16 .times. 102J/m2) induced maximal net release of performed mediators of 44.52 .+-. 6.6 to 58.01 .+-. 4.0% (mean .+-. SE). In contrast, irradiation in the presence of URO (50-5000 ng/ml) resulted in < 5% net release. [3H]Serotonin release induced by PP and irradiation was calcium-independent, and was not enhanced by phorbol 12-myristate 13-acetate, a known activator of protein kinase C. This release was suppressed by catalase, a scavenger of hydrogen peroxide. Furthermore, irradiation in the presence of PP, but not in the presence of URO, resulted in perturbation of cell membrane. Irradiation in the presence of PP also resulted in a maximal net release of generated mediators of 9.98 .+-. 3.5% (mean .+-. SE), whereas similar treatment in the presence of URO induced < 0.5% net release. These results suggested that the burning, stinging, erythema, and edema experienced by patients with erythropoietic protoporphyria following sun exposure, and the lack of such findings in patients with porphyria cutanea tarda, may be explained, at least in part, by the differential effects of PP and URO on mast cells.

Please choose payment method:

(PDF emailed within 0-6 h: $19.90)

Accession: 005155423

Download citation: RISBibTeXText

PMID: 3102621

DOI: 10.1111/1523-1747.ep12466140

Related references

Differential effect of protoporphyrin and uroporphyrin on mast cells correlation with clinical manifestations of erythropoietic protoporphyria and porphyria cutanea tarda. Clinical Researchpart 2: 662a, 1985

Effect of protoporphyrin uroporphyrin and irradiation on purified mast cells. Clinical Research: 156a, 1985

Demonstration of differential effects of cytokines on mast cells derived from murine bone marrow and peripheral blood mononuclear cells. Experimental Hematology 22(12): 1147-1155, 1994

Biosynthesis of porphyrins and related macrocycles. Part VII. Synthesis of specifically labelled (14 C1) uroporphyrin-III and of (10,14-13C2)-uroporphyrin-III. Conversion of the latter into (10,14-13C2)protoporphyrin-ix; Biosynthetic significance of its 13C nuclear magnetic resonance spectrum. Journal of the Chemical Society. Perkin Transactions 1 1976(3): 283-291, 1976

Dual effects of protoporphyrin and long wave ultraviolet light on histamine release from rat peritoneal and cutaneous mast cells. Journal of Immunology 144(11): 4327-4332, 1990

Histamine release from rodent and human mast cells induced by protoporphyrin and ultraviolet light: studies of the mechanism of mast-cell activation in erythropoietic protoporphyria. British Journal of Dermatology 122(4): 501-512, 1990

Role of uroporphyrin III as a precursor of protoporphyrin IX. Fed Proc 11(1): 280, 1952

Tetrapyrrole precursors of protoporphyrin IX. I. Uroporphyrin III. Journal of Biological Chemistry 196(2): 463-468, 1952

Mechanisms of photosensitization by protoporphyrin coproporphyrin and uroporphyrin. Molecular Aspects of Medicine 11(1-2): 109, 1990

Mast cell heterogeneity. Differential synthesis and expression of glycosphingolipids by mouse serosal mast cells as compared to IL-3-dependent bone marrow culture-derived mast cells before or after coculture with 3T3 fibroblasts. Journal of Immunology 145(5): 1463-1468, 1990

Reactive species involved in the photosensitization by protoporphyrin and uroporphyrin. Photochemistry & Photobiology 49(Suppl.): 48S, 1989

PKCtheta-responsiveness and differential transactivation activities of two isoforms of murine NFAT2 in mast cells. FASEB Journal 16(4): A695, 2002

A differential staining method for mast cells and eosinophils in murine intestine, liver and spleen. Parasitology Today 11(5): 194-196, 1995

Mechanism of phototoxicity of protoporphyrin and uroporphyrin on bovine corneal endothelium. Journal of Toxicology Cutaneous & Ocular Toxicology 13(1): 55-65, 1994

Reactive species involved in photosensitivity of protoporphyrin and uroporphyrin in an in vitro model. Photochemistry & Photobiology 53(Suppl.): 117S, 1991