Differential staining and chromatin packing of the mitotic chromosomes of the newt triturus cristatus

Rudak, E.; Callan, H.G.

Chromosoma 56(4): 349-362

1976


ISSN/ISBN: 0009-5915
DOI: 10.1007/bf00292955
Accession: 005158029

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Abstract
Mitotic metaphase chromosomes of cold-treated T. cristatus show a characteristic pattern of constrictions, most of which lie close, though not immediately adjacent, to the centromeres. The chromatin in these cold-induced constrictions stains intensely with Giemsa. Cold-treated spermatogonia show spiral structure throughout the metaphase chromatids; the packing of chromatin fibrils is much tighter in the constricted regions than elsewhere, and the gyres in the constricted regions are narrower and of shorter pitch.

Differential staining and chromatin packing of the mitotic chromosomes of the newt triturus cristatus