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Differential suppression of fsh and luteinizing hormone secretion by follicular fluid in the presence or absence of gonadotropin releasing hormone



Differential suppression of fsh and luteinizing hormone secretion by follicular fluid in the presence or absence of gonadotropin releasing hormone



Neuroendocrinology 38(3): 199-205



The differential role of porcine follicular fluid (pFF) in regulating FSH and luteinizing hormone (LH) release in vivo in situations of different gonadotropin-releasing hormone (GnRH) backgrounds was studied. In experiment 1, 2-wk ovariectomized rats injected i.v. with 4, 16 or 64 mg of protein from pFF, showed a dose-dependent suppression of FSH over time, with a maximal suppression to 40% of control values by 10 h. LH levels were slightly, but significantly elevated by the 2 lower doses, but not by the highest dose of pFF. In experiment 2, 64 mg pFF was superimposed (i.v. injection) in ovarietomized rats injected s.c. with a high dose of GnRH antagonist (500 .mu.g) 24 h earlier. The pFF suppressed FSH 35% below the level achieved in the absence of GnRH stimulation, with no effect on LH. In experiment 3, the rise in FSH secretion in acutely ovariectomized rats was shown to be inhibited by 8 or 32 mg pFF administered i.v. 3.5 h after surgery. Injection of GnRH (250 or 1,000 ng) 4.5 h after pFF could not overcome the inhibitory action of pFF on FSH, although non-pFF-treated controls responded in a dose-dependent fashion to GnRH stimulation. The expected LH response to GnRH was not affected by pFF, except in the group receiving 1000 ng GnRH and 8 mg pFF. In these rats, LH was enhanced in 1 trial, but suppressed in a replicate trial, illustrating the inconsistent effects on pFF on LH under conditions of high GnRH stimulation. These results demonstrate the existence of a component of FSH secretion which is independent of GnRH, but sensitive to the inhibitory effect of pFF, suggesting a site of action of the putative peptide hormone, folliculostatin, distal to the GnRH receptor. Also, pFF always suppresses FSH (due to the action of folliculostatin); its effect on LH depends on the dose and the state of GnRH stimulation, and is probably not a function of folliculostatin per se.

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Accession: 005158143

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