Differentiation in callus cultures of leaf of 2 species of trigonella
Sen, B.; Gupta, S.
Physiologia Plantarum 45(4): 425-428
1979
ISSN/ISBN: 0031-9317 DOI: 10.1111/j.1399-3054.1979.tb02607.x
Accession: 005158947
Callusing of leaf segment of T. corniculata L. and T. foenum-graecum L. was observed on Murashige and Skoog's (MS) medium and White's (W) medium supplemented with naphthalene acetic acid (NAA; 0.5 mg/l) and coconut milk (15% vol/vol). Root, shoot and development of isolated leaf occurred in the calli of both the species on MS medium containing NAA and coconut milk. Omission of coconut milk with subsequent addition of casein hydrolysate (CH) increased the number of differentiated organs per callus. In both the materials a higher differentiation rate was observed at low irradiance than at high irradiance or in complete darkness. Initially the cells were diploid but at the 5th passage the calli became mixoploid with predominantly diploid cell populations. The differentiated organs were diploid.