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Differentiation of canine coronavirus and porcine transmissible gastroenteritis virus by neutralization with canine porcine and feline sera



Differentiation of canine coronavirus and porcine transmissible gastroenteritis virus by neutralization with canine porcine and feline sera



Veterinary Microbiology 5(4): 283-290



Monospecific antisera were prepared in rabbits against canine coronavirus (CCV) and transmissible gastroenteritis virus of pigs (TGEV), and in 24 pigs and 3 cats against TGEV alone. Neutralizing antibody titers were higher for the immunizing than the heterologous virus, although cross-neutralization usually was detected. This confirmed that CCV and TGEV are distinct, but antigenically related coronaviruses. In sera from 41 dogs, CCV-neutralizing titers were on average 2.7-fold higher than TGEV-neutralizing titers, suggesting that CCV was the causal agent. Sera from 29 cats in colonies with feline infectious peritonitis (FIP) and known to contain TGEV-neutralizing antibody, were found to have titers 12.3-fold higher against CCV. The FIP virus (FIPV) is probably more closely related to CCV than TGEV, as judged by antigens involved in virus neutralization. Antisera to 2 isolates of bovine coronavirus, 3 isolates of hemagglutinating encephalomyelitis virus, 7 strains of avian infectious bronchitis virus and the 229E strain of human coronavirus all failed to neutralize CCV and TGEV. Thus CCV, TGEV and probably FIPV fall into a group of antigenically related agents, separable from other members of the family Coronaviridae, by both virus neutralization and immunofluorescence tests.

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Accession: 005159163

Download citation: RISBibTeXText

DOI: 10.1016/0378-1135(80)90027-9


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