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Differentiation of two serotypes of cucumber mosaic virus in japan by fab' 2 elisa with cross absorbed antibodies



Differentiation of two serotypes of cucumber mosaic virus in japan by fab' 2 elisa with cross absorbed antibodies



Berichte des Ohara Instituts fuer Landwirtschaftliche Biologie Okayama Universitaet 19(3): 149-158



Two serotypes of cucumber mosaic virus (CMV) designated Y and P types in Japan were tested against homologous and heterologous antibodies in F(ab')2 ELISA. The ELISA values of homologous reactions were higher than those in heterologous combinations. The use of cross-absorbed antibodies with heterologous serotypes as detecting antibodies was demonstrated to be valuable in differentiating two serotypes of CMV in F(ab')2 ELISA using crude sap from infected plants. One hundred and twenty-four isolates of CMV obtained from 38 plant species in 20 families collected in Japan were serotyped by this method. One hundred and fifteen isolates belonged to Y and 9 to P serotypes. All 3 strains isolated from Petasites japonicus were P type strains.

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Accession: 005160063

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