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Digestibility determination in fish using chromic oxide marking and analysis of contents from different segments of the gastro intestinal tract

, : Digestibility determination in fish using chromic oxide marking and analysis of contents from different segments of the gastro intestinal tract. Aquaculture 13(3): 265-272

Methods of feed digestibility determination in fish were reviewed. The indicator method, using chromic oxide, was judged most suitable but the best method of sampling feces was uncertain. Rainbow trout (Salmo gairdneri, Richardson) were fed an experimental diet containing 1% chromic oxide. Feces were collected by 2 stripping methods, and gut contents were obtained by dissection from 5 zones of the alimentary canal. Analyses of feed, feces and gut contents were made, and digestibility of protein, fat, carbohydrate, ash and gross energy calculated. Absorption occurs in all parts of the alimentary tract, and it is important to limit the stripping of feces for analysis in digestibility studies to the hindmost part of rectum, i.e., from the ventral fin to anus.

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