Direct exposure of postimplantation mouse embryos to 5-bromodeoxyuridine in vitro and its effect on subsequent chondrogenesis in the limbs

Agnish, N.D.; Kochhar, D.M.

Journal of Embryology and Experimental Morphology 36(3): 623-638


ISSN/ISBN: 0022-0752
PMID: 1034666
Accession: 005165326

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Treatment of 11th-day mouse embryos, in vitro, with BudR [5-bromodeoxyuridine] for 24 h was followed by organ culture of their forelimbs for 9 days in drug-free medium. A significant and dose-related inhibition of chondrogenesis in the limb explants was observed. BudR treatment of embryos at a time when their limb development was at an early stage of development, damaged the future course of limb differentiation. The rather low frequency of limb malformations after in vivo injection of teratological doses of BudR was due to only a small amount of the drug reaching the embryos.