Direct somatic embryoid formation on immature embryos of trifolium repens trifolium pratense and medicago sativa and rapid clonal propagation of trifolium repens

Maheswaran, G.; Williams, E.G.

Annals of Botany 54(2): 201-212


ISSN/ISBN: 0305-7364
Accession: 005166295

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By direct somatic embryogenesis in vitro a clone of aseptic plantlets can be raised from a single immature embryo of T. repens (white clover) within .apprx. 6 wk of pollination. Embryoids are induced directly from intact zygotic embryonic tissue on a culture medium containing 0.025 or 0.05 mg l-1 BAP [b-benzyl aminopurine] and 1.0 g l-1 yeast extract. Similar direct somatic embryogenesis was also achieved for T. pratense (red clover) and M. sativa (lucerne). Applications of embryo propagation by direct somatic embryogenesis are discussed, particularly in relation to multiple screening of host genotypes for analysis of host/pathogen and legume/Rhizobium interactions.