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Direct spectrophotometry of magnesium in serum after reaction with hexokinase ec 2.7.1.1 and glucose 6 phosphate dehydrogenase ec 1.1.1.49



Direct spectrophotometry of magnesium in serum after reaction with hexokinase ec 2.7.1.1 and glucose 6 phosphate dehydrogenase ec 1.1.1.49



Clinical Chemistry 31(5): 703-705



A simple method for determining Mg in serum [from humans] by using hexokinase (EC 2.7.1.1) and glucose-6-phosphate dehydrogenase (EC 1.1.1.49) was described. The method is based on determination of the reaction rate of hexokinase activated by Mg2+, which participates in the hexokinase reaction as the substrate in the form of a Mg .cntdot.ATP2- complex. The reaction rate is determined from the change in absorbance at 340 nm as NADPH is produced by the action of glucose-6-phosphate dehydrogenase. This simple and rapid spectrophotometric method does not require expensive instrumentation, but results correlate satisfactorily with those obtained by atomic absorption spectroscopy. Thus, this method gives a true value for Mg in serum, a value appreciably lower than that obtained by an earlier colimetric method, the Xylidyl Blue II method, which lacks specificity.

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Accession: 005166323

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