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Discontinuous polyacrylamide gel electrophoresis of cell wall proteinase from variants of streptococcus lactis

Discontinuous polyacrylamide gel electrophoresis of cell wall proteinase from variants of streptococcus lactis

Journal of Applied Bacteriology 58(3): 245-250

An electrophoretic procedure was developed for the separation of plasmid-coded cell wall proteinases from S. lactis NCDO strain 712. The pH and casein concentration within the electrophoresis rod during detection is more closely defined than in existing methods. The incubation conditions used with casein, (.apprx. pH 7 and 30.degree. C) may be suitable for the detection of a wider range of proteinases from other sources. Four proteinase bands present in the cell wall were detected in milk grown cells, but only 2 in those previously grown in a broth medium. No cell wall proteinases were detected in a proteinase deficient variant.

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Accession: 005167583

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DOI: 10.1111/j.1365-2672.1985.tb01456.x

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