+ Site Statistics
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on LinkedInFollow on LinkedIn

+ Translate

Displaceable binding of tritiated l glutamic acid to non receptor materials

Displaceable binding of tritiated l glutamic acid to non receptor materials

Life Sciences 38(12): 1089-1096

[3H]L-glutamic acid binding to microfuge tubes and glass was investigated in four buffers. Background binding to these materials was negligible, but was increased by centrifugation or suction in Tris-HCl and Tris-citrate buffer. This binding was much less or eliminated when HEPES-KOH, or Tris-acetate buffer was used instead. [3H]L-glutamate binding to microfuge tubes was inhibited by L- but not D-isomers of glutamate and aspartate. DL-2-amino-7-phosphono-heptanoic acid also did not inhibit the binding. Other compounds which showed low to moderate inhibition were: N-methyl-D-aspartate, quisqualate, L-glutamic acid diethyl ester, N-methyl-L-aspartate, kainate, and 2-amino-4-phosphonobutyrate. Binding was inhibited by denatured rat brain membranes. A protein-dependent [3H]glutamate binding was obtained with a repeatedly frozen-thawed membrane preparation when binding was done in Tris-acetate buffer. It is recommended that Tris-acetate or HEPES-KOH buffer should be used in the glutamate binding assay. If Tris-HCl or Tris-citrate buffer is used, appropriate control experiment should be done to correct for binding to microfuge tubes or glass fiber filters.

(PDF 0-2 workdays service: $29.90)

Accession: 005170335

Download citation: RISBibTeXText

Related references

Displaceable binding of [3H]l-glutamic acid to non-receptor materials. Life Sciences 38(12): 1089-1096, 1986

Binding of tritiated MK-801, NMDA-displaceable tritiated glutamate, tritiated glycine, tritiated spermidine, tritiated kainate and tritiated AMPA to regionally discrete brain membranes of the gerbil: A biochemical study. Neurochemistry International 22(1): 37-43, 1993

Synthesis optical resolution and radiolysis of amino acids of high specific activity tritiated 3 4 5 5 l ornithine tritiated 3 4 5 5 l arginine tritiated 3 4 dl glutamic acid and tritiated 3 4 4 dl glutamic acid. Journal of Labelled Compounds & Radiopharmaceuticals 23(5): 553-564, 1986

The binding properties and regional ontogeny for tritiated glutamic acid sodium independent and tritiated kainic acid binding sites in chick brain. International Journal of Developmental Neuroscience 4(4): 339-352, 1986

Effects of acromelic acid a on the binding of tritiated glutamic acid and tritiated kainic acid to synaptic membranes and on the depolarization at the frog spinal cord. Brain Research 504(2): 328-331, 1989

Effects of acromelic acid on the binding of tritiated glutamic acid and tritiated kainic acid to cell membranes from several types of tissues. Japanese Journal of Pharmacology 43(SUPPL): 263P, 1987

Effects of ascorbic acid on tritiated gamma aminobutyric acid and tritiated glutamic acid binding to rat brain cortical synaptosomes. Byulleten' Eksperimental'noi Biologii i Meditsiny 102(9): 288-289, 1986

Synthesis of doubled glutamic acid derivatives and their effect on the receptor binding of tritiated l glutamate. Khimiko-Farmatsevticheskii Zhurnal 21(6): 655-659, 1987

The sites of high affinity binding of tritiated l glutamic acid in human platelets a new type of thrombocyte receptor. Biokhimiya 53(5): 848-852, 1988

Human platelet dense granules improved isolation and preliminary characterization of tritiated serotonin uptake and tetrabenazine displaceable tritiated ketanserin binding. Life Sciences 46(24): 1755-1764, 1990

Effects of polyamine spider venom components on tritiated glutamic acid and tritiated glycine binding to rat cns membranes. Society for Neuroscience Abstracts 15(2): 1168, 1989

The effect of N-phthalamoyl-L-glutamic acid (PhGA), a novel selective NMDA receptor agonist, on the binding of tritiated L-glutamate with the synaptic membranes of human hippocampus. Byulleten' Eksperimental'noi Biologii i Meditsiny 113(6): 563-565, 1992

Excitatory amino acid receptors in the human cerebral cortex a quantitative autoradiographic study comparing the distributions of tritiated tcp tritiated glycine tritiated l glutamate tritiated ampa and tritiated kainic acid binding sites. Neuroscience 32(3): 587-608, 1989

Evidence for a displaceable non specific tritiated neurotensin binding site in rat brain. Naunyn-Schmiedeberg's Archives of Pharmacology 333(4): 400-405, 1986

Glutamic acid insensitive tritiated kainic acid binding in goldfish brain. Brain Research 571(1): 73-78, 1992