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Dna relatedness among selected strains of mycobacterium tuberculosis mycobacterium bovis mycobacterium bovis bcg mycobacterium microti and mycobacterium africanum



Dna relatedness among selected strains of mycobacterium tuberculosis mycobacterium bovis mycobacterium bovis bcg mycobacterium microti and mycobacterium africanum



International Journal of Systematic Bacteriology 35(2): 147-150



DNA of selected strains of the M. tuberculosis complex, including strains of M. tuberculosis, M. bovis, M. bovis BCG, M. microti and M. africanum, were isolated and analyzed spectrophotometrically. The genome MW and the guanine-plus-cytosine content of the DNA samples were .apprx. 1.9 .apprx. 109 and 62.9 mol%, respectively. DNA relatedness among the strains was studied by spectrophometric DNA hybridization and restriction endonuclease cleavage methods. All strains of the 4 species showed > 90% DNA relatedness, except for slightly lower levels (85-89%) of relatedness between M. microti and some strains of M. bovis, M. bovis BCG or M. africanum. The distribution patterns of the DNA fragments produced with restriction enzymes, such as EcoRI, EcoRV, BglII, BclI and KpnI, were indistinguishable upon agarose gel electrophoresis. In contrast, the levels of DNA relatedness between M. tuberculosis and other slowly growing mycobacteria, including M. nonchromogenicum, M. intracellulare, M. kansasii, M. avium, M. scrofulaceum, M. haemophilum, M. marinum and M. asiaticum, were significantly heterogeneous, ranging from 9-53%. The data indicate the extremely close evolutionary relationship among 4 species of the M. tuberculosis complex.

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