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Effect of 3 amino 1 2 4 triazole on the catalase activity of saccharomyces cerevisiae a new procedure for cytochrome c peroxidase ec 1.11.1.5 assay



Effect of 3 amino 1 2 4 triazole on the catalase activity of saccharomyces cerevisiae a new procedure for cytochrome c peroxidase ec 1.11.1.5 assay



Microbios 37(147): 29-36



Yeast cytochrome c peroxidase assay presents 2 different problems, namely electron donor oxidation by cytochrome c oxidase, and rapid decomposition of the substrate (H2O2). A new procedure was described for avoiding these difficulties by using o-dianisidine as electron donor and 3-amine-1,2,4-triazole for catalase inactivation. Liberation and solubilization kinetics of peroxidase are discussed.

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Accession: 005214554

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