Effect of cyclophosphamide on primary herpes simplex uveitis in rabbits

Oh, J.O.

IOVS Investigative Ophthalmology and Visual Science 17(8): 769-773


ISSN/ISBN: 0146-0404
PMID: 355184
Accession: 005230448

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Intracameral injection of the normal rabbit eye with 103 50% tissue culture infectious doses of type 1 herpes simplex virus (HSV) caused uveal inflammation, biphasic elevation of intraocular pressure (IOP) and virus growth in the ocular tissues. A series of i.m. injections of an immunosuppressive agent, cyclophosphamide (Cy), did not affect the ocular changes or the multiplication of virus in the uveal tissues in the early stage of the disease (days 1-6). During this period neither circulating antibody [Ab] nor macophage migration inhibition factor (MIF) against HSV appeared in control or Cy-treated animals, suggesting that the disease is initiated by an infectious process. After day 7, virus growth in the uveal tissues of the Cy-treated animals was much more abundant and more prolonged than in the tissues of the control eyes, but uveal inflammation in the Cy-treated animals was much milder and subsided earlier than in the control animals. The Cy treatment completely suppressed the 2nd rise of IOP which normally appears on day 7. These effects of Cy in the late period coincided with a significant suppression of circulating Ab and MIF, indicating that the late stage of the uveitis was mediated by immune mechanisms. Primary herpes simplex uveitis in the rabbit is dually mediated, first by the mechanisms of infection and later by the mechanisms of immunity.