Effect of ehrlich ascites tumor growth on lactate dehydrogenase ec activities in tissues and physiological fluids of tumor bearing mice

Bengtsson, G.; Andersson, G.

International Journal of Biochemistry 13(1): 53-62


ISSN/ISBN: 0020-711X
Accession: 005235480

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Tumor growth kinetics and the activity of lactate dehydrogenase (LDH) in body fluids and host tissues were studied during the Ehrlich ascites tumor growth. The rate of tumor cell death was determined by measuring the loss of 125I from mice bearing [5-125I]iodo-2'-deoxyuridine labeled tumor cells. The activity of LDH in serum increased progressively with increasing tumor mass during the 1st period of tumor growth; that of cell free ascites fluid exhibited a peak level during early plateau phase growth. The analysis of LDH activity in cell free ascites fluid revealed that the increased LDH activity could only partly be attributed to a release from dead tumor cells. A marked increase in host liver LDH activity was observed. The growing tumor induced an increase in RNA synthesis activity and Kupffer cell proliferation in the liver. The LDH activity in skeletal muscle decreased significantly with increasing tumor mass. The growing tumor exerts a marked effect on the metabolic activity of host tissues. An altered metabolism affects the cell membrane integrity of certain host tissues and causes a leakage of LDH molecules, which contributes to the increased extracellular LDH activity of tumor bearing animals.