EurekaMag.com logo
+ Site Statistics
References:
53,869,633
Abstracts:
29,686,251
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on LinkedInFollow on LinkedIn

+ Translate

Effect of elastase or histamine on single breath nitrogen washouts in the rat



Effect of elastase or histamine on single breath nitrogen washouts in the rat



Journal of Applied Physiology Respiratory Environmental & Exercise Physiology 52(1): 141-146



A method for performing single-breath N2 washouts (SBNW) in rats was developed and the test's usefulness was studied using 2 experimentally induced models of lung disease. Rats were anesthetized, intubated with oral tracheal and esophageal catheters, and tested by plethysmography. The SBNW expirogram was recorded during exhalation after inhaling 100% O2 from residual volume. The slope of phase III (slope III), closing volume (CV) and closing capacity (CC) were calculated. Changes in the SBNW expirogram were compared with changes in breathing pattern, dynamic and quasistatic lung mechanics, lung volumes and forced expiratory indices. Pre- and postinstillation tests were performed on rats treated with elastase or histamine and on untreated controls. The SBNW indices were altered at significance levels equal to those of other indices of lung function, and different patterns of change were induced by the two disease models. Elastase increased CV and CC, but slope III was unchanged. Other tests suggested loss of elastic recoil and expiratory flow limitation. Histamine increased slope III, but CV and CC were unchanged. Other tests suggested large airway constriction. The usefulness of the SBNW in rats is suggested, but the relationships between SBNW changes and underlying physiological phenomena remain to be defined.

(PDF emailed within 1 workday: $29.90)

Accession: 005235516

Download citation: RISBibTeXText



Related references

Effect of elastase or histamine on single-breath N2 washouts in the rat. Journal of Applied Physiology: Respiratory, Environmental and Exercise Physiology 52(1): 141-146, 1982

Cardiogenic oscillations and ECG phase relationship during single-breath nitrogen washouts performed in microgravity. Clinical & Investigative Medicine 18(4 SUPPL ): B134, 1995

Detection of uneven distribution of inspired gas in the lung and its clinical applications by single and multiple breath nitrogen washouts. Journal of Kyoto Prefectural University of Medicine 94(6): 533-554, 1985

Method for analyzing multiple breath nitrogen washouts. Medical & Biological Engineering & Computing 24(1): 83-90, 1986

Single-breath washouts in a rotating stretcher. Journal of Applied Physiology 90(4): 1415-1423, 2001

Single Breath Washouts in Exercise-Induced Asthma. Clinical Science 52(2): 27p.3-28p, 1977

Single breath washouts in exercise induced asthma. Clinical Science & Molecular Medicine 52(2): 27P-28P, 1977

Slope of phase III in multiple-breath nitrogen washouts and washing in steers. Pfluegers Archiv European Journal of Physiology 427(3-4): R61, 1994

Separation of helium and sulfur hexa fluoride in single breath washouts. Physiologist 20(4): 26, 1977

Structural chance of the acinus during growth assessed by single-breath washouts. European Respiratory Journal 8(SUPPL 19): 55S, 1995

Structural change of the acinus during growth assessed by single-breath tracer gas washouts. Pediatric Pulmonology 22(4): 230-235, 1996

Inhomogeneity of pulmonary ventilation during sustained microgravity as determined by single-breath washouts. Journal of Applied Physiology 76(4): 1719-1729, 1994

Paradoxical helium and sulfur hexafluoride single-breath washouts in short-term vs. sustained microgravity. Journal of Applied Physiology 82(3): 859-865, 1997

The effect of breath holding on the single breath nitrogen washout. American Review of Respiratory Disease 109(6): 693-694, 1974

Effect on single-breath washout and lung function of elastase-induced emphysema in rats. American Review of Respiratory Disease 148(3): 735-743, 1993