Effect of extracellular pH and inhibitors on the gluconeogenesis and ammoniagenesis relationship in rat kidney cortex slices
Strzelecki, T.; Rogulski, J.
Acta Biochimica Polonica 24(4): 253-260
1977
ISSN/ISBN: 0001-527X PMID: 24970 Accession: 005238258
Gluconeogenesis from glutamine, fumarate, pyruvate, glutamine plus fumarate and glutamine plus pyruvate, was generally higher at pH 7.1 than at pH 7.4 and 7.7, but ammoniagenesis did not depend on the pH of the medium in rat renal cortex slices. The intermediates of the Krebs cycle decreased ammonia formation from glutamine, raising gluconeogenesis at the same time. Arsenite, malonate, maleate, hydrazine and 2,4-dinitrophenol inhibited gluconeogenesis and simultaneously enhanced ammonia formation irrespective of the pH of the medium.