Section 6
Chapter 5,239

Effect of extracellular pH and inhibitors on the gluconeogenesis and ammoniagenesis relationship in rat kidney cortex slices

Strzelecki, T.; Rogulski, J.

Acta Biochimica Polonica 24(4): 253-260


ISSN/ISBN: 0001-527X
PMID: 24970
Accession: 005238258

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Gluconeogenesis from glutamine, fumarate, pyruvate, glutamine plus fumarate and glutamine plus pyruvate, was generally higher at pH 7.1 than at pH 7.4 and 7.7, but ammoniagenesis did not depend on the pH of the medium in rat renal cortex slices. The intermediates of the Krebs cycle decreased ammonia formation from glutamine, raising gluconeogenesis at the same time. Arsenite, malonate, maleate, hydrazine and 2,4-dinitrophenol inhibited gluconeogenesis and simultaneously enhanced ammonia formation irrespective of the pH of the medium.

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