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Effect of fluoroquinolones on Chlamydia trachomatis as revealed by electron microscopy

Effect of fluoroquinolones on Chlamydia trachomatis as revealed by electron microscopy

Nippon Ganka Gakkai Zasshi 92(6): 973-980

Fluoroquinolones, of laxacin in particular, are known to be effective in the treatment of Chlamydial infections of the eye. To clarify the mode of action of this agent, HeLa cells were infected with a Chlamydia trachomatis C-type strain, and cultivated for 48 hours. Then ofloxacin was added to the culture medium at a concentration of 2 .mu.g/ml for the next 24 hours. Specimens were then examined by electron microscopy in thin sections. In control experiments, in which ofloxacin was not used, inclusions contained a great number of initial bodies under fusion fission along with a small number of mature elementary bodies at 48-hour-culture, and a great number of elementary bodies with a small number of initial bodies at 72-hour culture. When ofloxacin was added for the last 24 hours, inclusions contained a small number of elementary bodies with a great number of initial-body-ghost-shadow after 72 hours culture. It was concluded that the agent destroys the initial bodies during their development, but it has little action on established elementary bodies.

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Accession: 005239608

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PMID: 3189082

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