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Effect of heat stable hemolysin from pseudomonas aeruginosa on toxoplasma gondii and host cells


, : Effect of heat stable hemolysin from pseudomonas aeruginosa on toxoplasma gondii and host cells. Jikeikai Medical Journal 34(3): 281-288

Peritoneal exudate containing Toxoplasma tachyzoites and host cells were treated with various concentrations of heat-stable hemolysin from Pseudomonas aeruginosa and different sensitivities of those Toxoplasma and host cells to the hemolysin were studied. Pseudomonas hemolysin could solubilize preferentially the host cells but streptolysin S, one of the most powerful bacterial hemolysins, could not. The action of Pseudomonas hemolysin is considered to be detergent-like, but there existed marked difference in viability of the tachyzoites treated with hemolysin and Triton X-100. The latter decreased the viability drastically while the former did so gradually. The hemolysin solubilized brain tissue without damage to Toxoplasma cysts when treated for a short time. Pseudomonas hemolysin may be useful for recovery of viable T. gondii parasite from tissues.

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Related references

Makioka, A.; Kobayashi, A.; Matsuura, M.; Homma, J.Y., 1986: An electron microscope study on Toxoplasma gondii with heat stable hemolysin from Pseudomonas aeruginosa. T. gondii tachyzoites were isolated from mice 2 days after infection with the RH strain and were purified with 8 units (one unit represents the amount needed to lyse 1 ml of 1% RBCs) of a P. aeruginosa haemolysin; The tachyzoites were then treated...

Makioka, A.K.bayashi, A.M.tsuura, M.H.mma, J., 1986: An electron microscopic study on Toxoplasma gondii treated with heat-stable hemolysin from Pseudomonas aeruginosa. Japanese journal of parasitology 35(2): 149-151

Makioka, A.; Kobayashi, A.; Matsuura, M.; Homma, JY., 1986: An electron microscope study on Toxoplasma gondii treated with heat-stable hemolysin from Pseudomonas aeruginosa. Japanese Journal of Parasitology, 352: 149-151

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