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Effect of hemolymph and nervous tissue components on the in vitro development of flight muscle myoblasts of manduca sexta



Effect of hemolymph and nervous tissue components on the in vitro development of flight muscle myoblasts of manduca sexta



Differentiation 30(2): 92-97



Flight-muscle myoblasts from pupae of Manduca sexta were grown in the presence of either hemolymph or nerve extract. The cells exhibited distinctly different growth patterns: the addition of hemolymph yielded a branched network consisting of short, thin myofibers, while the addition of nerve extract resulted in parallel, extended fiber arrays. Spontaneous contractions were frequently observed. Other medium supplements (muscle extract, mouse-liver extract, .beta.-ecdysone) had no detectable effect on myoblast development. Electron micrographs of explants grown in hemolymph revealed the presence of only a few myofibrils with a parallel filament arrangement but without cross striation, whereas explants grown with nerve extract exhibited cross striation within the voluminous filament arrays.

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Accession: 005243353

Download citation: RISBibTeXText

DOI: 10.1111/j.1432-0436.1985.tb00519.x


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