Effect of hydrocortisone and cholesterol on the penetration of succinate in rat liver mitochondria

Vol'skii, G.G.; Burgova, M.P.; Velichko, E.V.

Vestnik Leningradskogo Universiteta Biologiya 3: 75-81

1978


Accession: 005244763

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Abstract
The influence of hydrocortisone and cholesterol on the penetration of succinate into rat liver mitochondria was studied in vitro. Two independent methods were used, namely, direct determination of accumulation of labeled 2,3-14C-succinate by mitochondria and measurement of reduction of intramitochondrial flavine and pyridine nucleotides by exogenous succinate using changes in intensity of their fluorescense. Both methods demonstrated similar effect of hydrocortisone and cholesterol on the transport process studied which is expressed in the decrease of mitochondria radioactivity and of reduction of nucleotides. The membraneous character of hormone action leading to the inhibition of succinate transport through the mitochondrial membrane is suggested.