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Effect of interleukin 2 on cyto toxic effectors 1. short term culture of cyto toxic effectors and in vivo anti tumor activity of cultured effectors isolated from the tumor site

Effect of interleukin 2 on cyto toxic effectors 1. short term culture of cyto toxic effectors and in vivo anti tumor activity of cultured effectors isolated from the tumor site

International Journal of Cancer 30(5): 625-632

The effects of interleukin 2 (IL2) on the in vitro and in vivo activity of cytotoxic T cells have been studied. IL2 was produced by W/Fu rat spleen cells cultured with concanavalin A. The IL2 thus prepared gave an optimal T-cell growth-promoting effect at a concentration of 5-20% equivalents of the original preparation. In the primary, syngeneic mixed lymphocyte/tumor cell cultures (MLTC) against FBL-3 [murine leukemia] tumor cells, the addition of IL2 failed to generate a cytotoxic response. The cytotoxic response could be generated in MLTC by addition of exogenous macrophages. IL2 could maintain the growth of preformed cytotoxic T cells for 3-5 wk. These cytotoxic T cells were generated by in vitro sensitization (MLC or MLTC) or by in vivo sensitization of B6 mice against a syngeneic tumor FBL-3. In short-term cultures, augmentation of the cytotoxic activity was seen after 10 day's culturing with IL2. The antigenic specificity of the cytotoxic reaction was altered after 28-35 days in culture, and the effectors broadly reactive. When growing a nonadherent population of lymphocytes isolated from FBL-3 ascites tumor, supplementation with IL2 selectively promoted the growth of a T-cell population, resulting in the elimination of the contaminating tumor cells. These purified T cells were highly cytotoxic for FBL-3 cells in vitro and also possessed strong in vivo anti-tumor activity against FBL-3 cells in the adoptive transfer experiments. Short-term culture (2-3 wk) in IL2 promotes the growth of T cells and augments their cytotoxic activity with the appropriate antigenic specificity. IL2 also promoted the selective growth of T cells isolated from tumor site and these T cells showed augmented in vitro and in vivo anti-tumor activity.

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