Effect of kinetin on the transcription activity of chromatin from cucumber cucumis sativus cultivar monastyrski cotyledons
Waliszewska Wojtkowiak, B.; Schneider, J.; Szweykowska, A.
Biochemie und Physiologie der Pflanzen (BPP) 180(6): 413-422
Three-day-old etiolated seedlings of C. sativus L. cv. Monastyrski were sprayed with water or 0.1 mM kinetin solution, which led to an enhancement of expansion growth of the cotyledons. After 24 h in darkness, the cotyledons were harvested, chromatin was isolated and its transcription activity tested. Chromatin from kinetin-treated cotyledons exhibited higher activity, both endogenous and supported with exogenous Escherichia coli RNA polymerase, which pointed to a higher DNA template activity as compared with the untreated control. Addition of kinetin to the reaction mixture for RNA synthesis in vitro, as well as the presence of kinetin during homogenization of the cotyledons, had no effect on the transcription activity of the chromatin. Kinetin inhibited elongation growth of the hypocotyls. Chromatin isolated from kinetin-treated hypocotyls displayed lower activity with respect to the untreated control. Also the presence of kinetin during hypocotyl homogenization led to a reduction of the transcriptional activity of the chromatin.