Effect of light intensity during growth on physiological and photosynthetic characteristics of cell suspension cultures of nicotiana tabacum cultivar xanthi

Nato, A.; Hoarau, J.; Bourdu, R.

Biology of the Cell 47(2): 213-218


ISSN/ISBN: 0248-4900
Accession: 005250634

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This work attempted to study the influence of 2 different light intensities (3.9 and 39 W.m-2) on the physiological and growth parameters of green cell suspensions of N. tabacum (cv. Xanthi) cultured in sucrose-enriched medium. The high light intensity reduced the doubling time from 3.4 to 2.4 days and stimulated the biomass production and chlorophyll accumulation 2-fold. Comparative measurements of CO2 content in the culture flasks at 3.9 and 39 W.m-2 showed a specific growth phase under high light where a good photosynthetic efficiency of cells allowed fixation of respiratory CO2. Thus, 90% of sucrose carbon was recovered in dry weight. The 2-3 times higher rates of O2 evolution and CO2 fixation via ribulose-1,5-bisphosphate carboxylase (RUBPCase) in cells at 39 W.m-2 support the hypothesis of an autotrophic growth potentiality of green tobacco cells. Under both growth conditions, the respiratory peak and the high phosphoenol-pyruvate carboxylase (PEPCase) capacity were concomitant with an active process of soluble protein synthesis.