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Effect of migration inhibitory factor on macrophage sub populations

Effect of migration inhibitory factor on macrophage sub populations

Indian Journal of Experimental Biology 18(2): 106-111

Peritoneal exudate cells collected from C57B1/6J (H-2b, M 1 sb) mice were fractionated into 5 subpopulations by discontinuous buoyant density gradient centrifugation. Macrophages banded at interfaces of 7/14, 14/18, 18/22, 22/27 and 27/30% bovine serum albumin were designated as subpopulation 1, 2, 3, 4 and 5 and the relative distributions were 1, 4, 15, 43 and 31%, respectively. These cells were mostly macrophages except subpopulation 5, which contained 30-40% lymphocytes. Biological parameters evaluated with subpopulations 2-5 showed positive reactions with varying responses. Maximum phagocytosis of 3H-labeled Escherichia coli was shown by subpopulation 4 and minimal activity by subpopulation 2. Mouse macrophage migration inhibition factor (MIF) induced a significant level of inhibition in the migration of macrophage subpopulations 3, 4 and 5. Subpopulation 2 macrophages exhibited very sluggish cell migration in control agarose droplet migration inhibition assay and an insignificant migration inhibition in the presence of MIF. All macrophage subpopulations, viable and non-viable, bound 125I-MIF at C, the maximum MIF-binding being obtained with subpopulation 2. MIF-binding at C was lower than those at C for all subpopulations. Exposure to MIF elicited a significant increase in 3H-2-deoxy-D-glucose transport across the cell membrane of subpopulation 2 and in acid phosphatase activity of subpopulation 4.

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Accession: 005254265

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PMID: 6995281

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