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Effect of molybdate on the molecular organization of the estrogen receptor system of porcine uterus



Effect of molybdate on the molecular organization of the estrogen receptor system of porcine uterus



Journal of Biochemistry 98(4): 1083-1090



Molybdate was shown to have complex effects in modulating the molecular organization of the constituents of the estrogen receptor (ER) system of porcine uterus. We showed previously the presence of one basic ER molecule (vero-ER) (sedimentation coefficient, 4.5S; Stokes radius, 44 A) and ER-binding factors (ERBFs) ["8S" ER-forming factor ("8S" ER-FF), (component A) .cntdot. (component B)6; "6S ER-FF, (component B)6; "5S" ER-FF, component A] in the porcine uterus [Fukai, F. and Myrayama, A. (1981) J. Biochem. 95, 1697-1704]. Molybdate regulates the specific interaction of vero-ER with ERBFs in a complex way. The apparent Kd value (6.7 .times. 10-10 M) of vero-ER with "8S" ER-FF in the presence of molybdate (30 mM) was decreased remarkably as compared with that (2.7 .times. 10-9 M) in the absence of molybdate. In contrast, the apparent Kd value (3.7 .times. 10-9 M) of vero-ER with "5S" ER-FF observed in the presence of molybdate (30 mM) was increased over ten-fold as compared with that in the absence of molybdate. Meanwhile, the affinity (Kd, 5 .times. 10-9 M) of vero-ER for "6S" ER-FF was scarcely influenced by molybdate. These results reveal the mechanism by which molybdate selectively stabilizes "8S" ER. Molybdate further affected the molecular constitution of ERBFs. The dissociation of "8S" ER-FF into component A and component B, which takes place under hypertonic (0.4 M KCl) conditions at higher temperature (25.degree. C), was suppressed almost completely by molybdate (30 mM). The previously reported inhibiting effect of molybdate on the formation of "5S" ER can be attributed to both the inhibition of the formation of "5S" ER-FF and to the decrease of the affinity of vero-ER for "5S" ER-FF. Molybdate did not influence the affinity of ER for estradiol.

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Accession: 005254798

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PMID: 4077842



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