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Effect of monensin on fluid phase and receptor mediated endocytosis by rat hepatocyte monolayers

Effect of monensin on fluid phase and receptor mediated endocytosis by rat hepatocyte monolayers

Biochemical & Biophysical Research Communications 152(3): 1275-1281

We have investigated the effect of monensin on the uptake of sucrose and sucrose-asialofetuin by rat hepatocyte monolayers and on the intracellular of these molecules. Endocytosis of surcose is not affected by monensin while that of sucrose-asialofetuin is markedly inhibited. Monensin causes a decrease of galactosyltransferase, a Golgi membrane enzyme without affecting dipeptidylpeptidase IV a plasma membrane enzyme and three lysosomal hydrolases. The inhibition of sucrose-asialofetuin uptake is proportional to the reduction of galactosyltransferase activity. Differrential and isopycnic centrifugation results indicate that monensin at a concentration (10 .mu.mol/l) that markedly decreases sucrose-asialofetuin uptake does not prevent the transfer of sucrose and sucrose-asialfetuin from endosomes to lysosomes.

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Accession: 005254864

Download citation: RISBibTeXText

PMID: 2454103

DOI: 10.1016/s0006-291x(88)80423-6

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