+ Site Statistics
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on LinkedInFollow on LinkedIn

+ Translate

Effect of mutations affecting sodium proton antiport activity on tumorigenic potential of hamster lung fibroblasts

Effect of mutations affecting sodium proton antiport activity on tumorigenic potential of hamster lung fibroblasts

Journal of Cellular Biochemistry 36(3): 249-260

Mutants unable to regulate intracellular pH through the Na+:H+ antiport system were found to evolve tumors less frequently than wild-type CCL39 hamster lung fibroblasts, after transplantation in athymic nude mice. When rare tumors arose, they comprised cells which were transformed in vitro, but which upon retransplantation grew at a lower rate than tumors cells originating from CCL39 cells. Both parental and mutant cells became transformed after transfection of the activated Harvey ras oncogene, but transfectants derived from the mutants had a weaker tumorigenic potential. These results suggest that transformed characteristics can be acquired independently from the Na+:H+ antiporter. However, the presence of this system provides a selective growth advantage when cells are confronted with natural environments, as it occurs during the expansion of tumors in a host.

(PDF emailed within 1 workday: $29.90)

Accession: 005255386

Download citation: RISBibTeXText

Related references

Effect of mutations affecting Na+: H+ antiport activity on tumorigenic potential of hamster lung fibroblasts. Journal of Cellular Biochemistry 36(3): 249-260, 1988

Biochemical characterization of the amiloride sensitive sodium proton antiport in chinese hamster lung fibroblasts. Journal of Biological Chemistry 258(6): 3503-3508, 1983

Cytotoxic t lymphocyte ctl mediated cytolysis proceeds in the absence of sodium proton antiport activity regulation of cytosolic ph by the sodium proton antiport in a cloned ctl. Cellular Immunology 135(1): 208-221, 1991

Sodium proton antiport activity in skin fibroblasts from blacks and whites. Hypertension (Dallas) 15(2): 140-145, 1990

Sodium/proton exchanger activity in Chinese-hamster lung fibroblasts Real-time detection using microphysiometry. FASEB Journal 8(4-5): A21, 1994

Intracellular pH and sodium, proton antiport activity of cultured skin fibroblasts from diabetics. Kidney International 42(5): 1184-1190, 1992

Proliferation and sodium-proton antiport activity in human fibroblasts from type I diabetic patients with nephropathy. American Journal of Hypertension 6(2): 170-173, 1993

Simvastatin affects ph regulation of sv40 transformed mrc5 fibroblasts by inhibition of sodium proton antiport activity. British Journal of Pharmacology 105(PROC SUPPL APRIL): 203P, 1992

Sodium proton antiport activity and cell growth in cultured skin fibroblasts of iddm patients with nephropathy. Diabetes 41(10): 1239-1246, 1992

A specific mutation abolishing sodium ion hydrogen ion antiport activity in hamster fibroblasts precludes growth at neutral and acidic ph. Proceedings of the National Academy of Sciences of the United States of America 81(15): 4833-4837, 1984

Enhanced growth and sodium-proton antiport activity response to serum in cultured fibroblasts of diabetic patients with nephropathy. Acta Diabetologica 29(3-4): 178-181, 1992

Blockade of the sodium proton antiport abolishes growth factor induced dna synthesis in fibroblasts structure activity relationships in the amiloride series. Journal of Biological Chemistry 259(7): 4313-4319, 1984

The effect of acute and chronic sodium chloride loading on lymphocyte sodium-proton antiport activity in healthy human subjects. European Journal of Clinical Investigation 23(SUPPL 1): A41, 1993

Effects of intracellular and extracellular proton and sodium ion concentrations on sodium ion proton antiport activity in the lacrimal gland acinar cells. Pfluegers Archiv European Journal of Physiology 417(4): 382-390, 1990

Racial differences in fibroblasts sodium proton antiport. Pediatric Research 23(4 PART 2): 538A, 1988