+ Site Statistics
References:
52,654,530
Abstracts:
29,560,856
PMIDs:
28,072,755
+ Search Articles
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ PDF Full Text
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Translate
+ Recently Requested

Effect of nitrogen to sulfur ratio on the incorporation of sulfur 35 into the thio amino acids of the microbial protein in an in vitro rumen system



Effect of nitrogen to sulfur ratio on the incorporation of sulfur 35 into the thio amino acids of the microbial protein in an in vitro rumen system



Journal of Nuclear Agriculture and Biology 7(2): 46-50



Three fistulated Tharparkar cows and 3 fistulated Murrha buffaloes, fed with 3 isonitrogenous urea based concentrate mixtures with N:S ratios of 20:1, 10:1 and 5:1 along with wheat straw, were used as donors or rumen inoculum for an in vitro experiment. The incubation vessels contained either of the 3 urea based purified substrates of N:S ratios (20:1, 10:1 and 5:1). Three .mu.Ci of 35S labeled Na2SO4 was added to each incubation vessel. At the end of 24 h incubation, the proteins were precipitated with TCA. The proteins were further hydrolyzed for the estimation of thioamino acids, i.e., cystine and methionine. 35S radioactivity in the protein precipitate and also in the cystine and methionine fraction was measured by liquid scintillation. The percentage incorporation of 35S into protein precipitate, the ratio of 35S activity/100 mg protein precipitate N and the net increase in the protein precipitate (mg/24 h) were all significantly higher (P < 0.01) with N:S ratio 10:1 than with N:S ratio 20:1. The percentage of 35S activity in the protein precipitate due to cystine and methionine, the specific radioactivity of 35S labeled cystine and methionine (cpm .times. 106/mg) and concentration of these 2 amino acids (mg/100 mg protein precipitate N) were also significantly higher (P < 0.01) with N:S ratio 10:1 and 5:1 than with N:S ratio 20:1. The dietary N:S ratio of 10:1 is optimum for the maximum protein add thioamino acid synthesis in the rumen. The buffalo rumen microbes synthesize more cystine and methionine and consequently more proteins than the microbes in cow rumen.

(PDF emailed within 1 workday: $29.90)

Accession: 005257077

Download citation: RISBibTeXText


Related references

The effect of amino acids and sulfur compounds on the utilization of non-protein nitrogen by rumen microorganisms in vitro, with special remark to L-methionine. Japanese Journal of Zootechnical Science 45(9): 494-501, 1974

Impact of immune system stimulation and sulfur amino acid intake on urinary sulfur excretion and whole body nitrogen to sulfur balance ratio in pigs. Canadian Journal of Animal Science 89(1): 153-153, 2009

Ratio of nitrogen and sulfur in urine as an index of intake of sulfur amino acids in a group of preschool children. Roczniki Panstwowego Zakladu Higieny 20(4): 449-455, 1969

Effect of force feeding of protein-free diets supplemented with sulfur-containing amino acids and sulfur-compounds on liver fat and urinary nitrogen. Agricultural and Biological Chemistry 39(3): 651-656, 1975

Location of carrageenan and incorporation of sulfur 35 labeled sulfur into sulfur amino acids in the red alga chondrus crispus. Hydrobiologia 116-117: 488-492, 1984

Effect of various treatments of protein on rumen metabolism and incorporation of sulfur 35 in the microbial protein. Journal of Nuclear Agriculture & Biology 9(4): 151-155, 1980

Sulfur amino acid nutrition during molt in the white-crowned sparrow. 2. Nitrogen and sulfur balance in birds fed graded levels of the sulfur-containing amino acids. Condor, 863: 324-332, 1984

Urinary sulfur excretion and the nitrogen/sulfur balance ratio reveal nonprotein sulfur amino acid retention in piglets. Journal of Nutrition 133(3): 766-772, 2003

Sulfur amino acid nutrition during molt in the white crowned sparrow zonotrichia leucophrys gambelii 2. nitrogen and sulfur balance in birds fed graded levels of the sulfur containing amino acids. Condor 86(3): 324-332, 1984

Effect of nitrogen phosphorus and sulfur on nitrogen sulfur ratio oil and protein content of brassica juncea l. Indian Journal of Agricultural Chemistry 21(1-2): 51-56, 1988

Effect of nitrogen/sulfur ratio and cellulose/starch ratio on the microbial protein synthesis and digestibilities of energy source. Korean Journal of Dairy Science 10(4): 151-158, 1988

Sulfur fertilization effects on the constancy of the protein nitrogen to protein sulfur ratio in low and high sulfur accumulating crops. Agronomy Journal 74(3): 415-418, 1982

Effect on rumen bacteria of methionine hydroxy analog and sulfur-containing amino acids, in vitro. Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine 142(2): 670-674, 1973

The stimulatory effect of the organic sulfur supplement, mercaptopropane sulfonic acid on cellulolytic rumen microorganisms and microbial protein synthesis in cattle fed low sulfur roughages. Animal 3(6): 802-809, 2009

Effect of colloidal sulfur on nitrogen, amino acids and sulfur metabolism, and on the wool productivity of sheep. 1981