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Effect of nitrogen to sulfur ratio on the incorporation of sulfur 35 into the thio amino acids of the microbial protein in an in vitro rumen system

Effect of nitrogen to sulfur ratio on the incorporation of sulfur 35 into the thio amino acids of the microbial protein in an in vitro rumen system

Journal of Nuclear Agriculture and Biology 7(2): 46-50

Three fistulated Tharparkar cows and 3 fistulated Murrha buffaloes, fed with 3 isonitrogenous urea based concentrate mixtures with N:S ratios of 20:1, 10:1 and 5:1 along with wheat straw, were used as donors or rumen inoculum for an in vitro experiment. The incubation vessels contained either of the 3 urea based purified substrates of N:S ratios (20:1, 10:1 and 5:1). Three .mu.Ci of 35S labeled Na2SO4 was added to each incubation vessel. At the end of 24 h incubation, the proteins were precipitated with TCA. The proteins were further hydrolyzed for the estimation of thioamino acids, i.e., cystine and methionine. 35S radioactivity in the protein precipitate and also in the cystine and methionine fraction was measured by liquid scintillation. The percentage incorporation of 35S into protein precipitate, the ratio of 35S activity/100 mg protein precipitate N and the net increase in the protein precipitate (mg/24 h) were all significantly higher (P < 0.01) with N:S ratio 10:1 than with N:S ratio 20:1. The percentage of 35S activity in the protein precipitate due to cystine and methionine, the specific radioactivity of 35S labeled cystine and methionine (cpm .times. 106/mg) and concentration of these 2 amino acids (mg/100 mg protein precipitate N) were also significantly higher (P < 0.01) with N:S ratio 10:1 and 5:1 than with N:S ratio 20:1. The dietary N:S ratio of 10:1 is optimum for the maximum protein add thioamino acid synthesis in the rumen. The buffalo rumen microbes synthesize more cystine and methionine and consequently more proteins than the microbes in cow rumen.

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Accession: 005257077

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