EurekaMag.com logo
+ Site Statistics
References:
53,869,633
Abstracts:
29,686,251
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on LinkedInFollow on LinkedIn

+ Translate

Effect of plasma lipoproteins on cholesterol accumulation in macrophages comparison of lipoproteins from normal and homozygous familial hypercholesterolemic subjects



Effect of plasma lipoproteins on cholesterol accumulation in macrophages comparison of lipoproteins from normal and homozygous familial hypercholesterolemic subjects



Atherosclerosis 65(3): 207-214



Total cholesterol (TC) content of mouse peritoneal macrophages (MPM) increased when incubated with increasing concentrations of normal low density (N-LDL) or very low density (N-VLDL) lipoprotein. Incubation with increasing concentrations of normal high density lipoprotein (N-HDL) caused a decrement in cellular mass of TC in MPM. Incubation of MPM with serum from normal subjects as well as from subjects with homozygous familial hypercholesterolemia (HFH) resulted in a 25% increment in cellular mass of TC, due to an increment in both free cholesterol (FC) and cholesteryl ester (CE) fractions. Accumulation of TC in MPM, due mainly to elevation of CE, was observed when the macrophages were incubated in the presence of LDL or VLDL derived from either group of subjects. N-LDL caused a higher increment in cellular CE compared to HFH-LDL. However, the presence of HFH-VLDL in the medium caused elevation in the cellular TC and CE content to a higher level than did N-VLDL. The presence of N-HDL as well as of HFH-HDL in the medium resulted in a similar decrement in the cholesterol content of MPM. The decrement was expressed in both FC and CE fractions. The present study shows different abilities of normal and HFH plasma lipoproteins to cause cholesterol accumulation in MPM.

(PDF emailed within 0-6 h: $19.90)

Accession: 005262215

Download citation: RISBibTeXText

PMID: 3619986

DOI: 10.1016/0021-9150(87)90036-0



Related references

Effect of cyclic AMP on the metabolism of low density lipoproteins and cholesterol in fibroblasts of normal subjects and subjects with homozygous type familial IIa hypercholesterolemia. Bulletin de L'academie Nationale de Medecine 167(7): 737-744, 1983

Metabolism of low-density lipoproteins by cultured hepatocytes from normal and homozygous familial hypercholesterolemic subjects. Biochimica et Biophysica Acta 876(3): 646-657, 1986

Familial hypercholesterolaemia : effect of low density lipoproteins on cholesterol synthesis in lymphocytes from homozygous and heterozygous subjects. Archives Internationales de Physiologie et de Biochimie 91(3): 179-185, 1983

Familial hypercholesterolaemia: effect of low density lipoproteins on esterification of cholesterol in lymphocytes from homozygous and heterozygous subjects. Archives Internationales de Physiologie et de Biochimie 95(1): 75-80, 1987

Cholesterol accumulation in J774 macrophages induced by triglyceride-rich lipoproteins. Comparison of very low density lipoprotein from subjects with type III, IV, and V hyperlipoproteinemias. Arteriosclerosis and Thrombosis 11(2): 221-233, 1991

Plasma lipids and lipoproteins after low and high cholesterol diets in hypercholesterolemic subjects. Rivista di Scienza dell'Alimentazione 24(1): 51-58, 1995

Familial apolipoprotein CII deficiency: plasma lipoproteins and apolipoproteins in heterozygous and homozygous subjects and the effects of plasma infusion. European Journal of Clinical Investigation 11(1): 69-76, 1981

Hepatic Golgi lipoproteins: precursors to plasma lipoproteins in hypercholesterolemic rats Low density lipoproteins. Journal of lipid research 23(7): 962-971, 1982

Platelet-modified low-density lipoproteins: studies in normal subjects and in patients with homozygous familial hypercholesterolemia. Clinical Biochemistry 20(2): 91-95, 1987

Characterization of hepatic low density lipoprotein binding and cholesterol metabolism in normal and homozygous familial hypercholesterolemic subjects. Journal of Clinical Investigation 73(2): 429-436, 1984

Uptake and degradation of high density lipoprotein: comparison of fibroblasts from normal subjects and from homozygous familial hypercholesterolemic subjects. Journal of Lipid Research 19(5): 644-653, 1978

Studies on the characterization of human serum lipoproteins separated by ultracentrifugation in a density gradient. I. Serum lipoproteins in normal, hyperthyroid and hypercholesterolemic subjects. American Journal of Clinical Nutrition 9: 24-40, 1961

Plasma lipoproteins in familial lecithin: cholesterol acyltransferase deficiency: physical and chemical studies of low and high density lipoproteins. Journal of Clinical Investigation 50(5): 1131-1140, 1971

Cholesterol metabolism in the genetically hypercholesterolemic (RICO) rat. II. A study of plasma lipoproteins and effect of dietary cholesterol. Biochimica et Biophysica Acta 960(3): 382-389, 1988

Effect of cholesterol esterase on low density lipoproteins accumulation of cholesterol by cultivated cells in a medium containing modified lipoproteins. Voprosy Meditsinskoi Khimii 35(3): 47-51, 1989