Effect of potential cytostatic and immune modulating chemicals on the plasma membrane of red blood cells as revealed by osmotic hemolysis cell electrophoresis and scanning electron microscopy

Augsten, K.; Peschke, T.; Wohlrabe, K.

Experimentelle Pathologie 17(7-8): 380-386


ISSN/ISBN: 0014-4908
Accession: 005263230

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The influence on red blood cells (RBC) of 2 cancerostatic-(Zimet 3106 and Zimet 3393) and 2 immunomodulating agents (Zimet 3164 and Zimet 86/76) administered s.c. or i.p. in case of the radiolabeled N-mustard compounds to mice, was studied by means of measurements of the radiolabeled compounds in blood, by cell electrophoresis, filipin-induced hemolysis of pretreated RBC and by scanning electron microscopy [SEM]. The portion of doses applied which bound to RBC-surface decreased in the sequence of ZIMET 3106, ZIMET 3164, ZIMET 3393 and ZIMET 86/76. The highest reduction of electrophoretic mobility and protection from flilipin-induced hemolysis of pretreated RBC was found with ZIMET 3164. In both tests ZIMET 86/76 proved to be ineffective. These findings were in good agreement with the modifications demonstrated in pretreated RBC after filipin-induced hemolysis by means of SEM. An N-mustard specific interaction between cholesterol of the membrane and/or other membrane constituents was indicated. These fundamental differences found in binding affinity, surface-charges and membrane interactions caused by chemically similar compounds cannot be attributed merely to the N-mustard group.