Effect of pre incubation with different concentrations of luteinizing hormone releasing hormone on subsequent luteinizing hormone release caused by supramaximally active amounts of luteinizing hormone releasing hormone role of luteinizing hormone releasing hormone induced protein synthesis

De-Koning, J.; Van-Dieten, J.A.M.J.; Van-Rees, G.P.

Life Sciences 21(11): 1621-1628


ISSN/ISBN: 0024-3205
Accession: 005263367

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Anterior pituitary glands from intact diestrous female rats were incubated for 2 consecutive periods of 3 h. During the 1st period various submaximally active amounts of luteinizing hormone-releasing hormone (LH-RH) were added to the media, whereas during the 2nd period a supramaximally active concentration of LH-RH was present. When during the 2nd incubation period protein synthesis was inhibited by cycloheximide, the amount of luteinizing hormone (LH) released during that period was positively correlated to the concentration of LH present during the 1st incubation period. This relationship was not seen when cycloheximide was absent, or when cycloheximide was present throughout both periods. Total LH was not affected by LH-RH; thus no effect of LH-RH on LH synthesis was observed. The amounts of protein synthesized by the pituitary glands in response to the different amounts of LH-RH during the 1st incubation period can constitute a limiting factor for the response to the supramaximally active amount of LH-RH added during the 2nd incubation period.