EurekaMag.com logo
+ Site Statistics
References:
52,725,316
Abstracts:
28,411,598
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Effect of prolactin on rubidium 86 uptake potassium content and tritium labeled ouabain binding of lactating rabbit mammary tissue


Journal of Physiology (Cambridge) 334: 1-18
Effect of prolactin on rubidium 86 uptake potassium content and tritium labeled ouabain binding of lactating rabbit mammary tissue
Ouabain-sensitive 86Rb+ uptake in slices of lactating rabbit mammary gland significantly increased after 20 min or 1 h of incubation with ovine prolactin (NIH-P-S12; 1 .mu.g/ml). Total K+ content of the tissue significantly increased at 20 min of incubation with prolactin. Neither vasopressin nor oxytocin, at concentrations of 2, 20 or 40 .mu.IU/ml, had a significant effect on ouabain-sensitive 86Rb+ uptake or total K+ of the tissue after 30 min or 1 h of incubation. Tissue slices incubated in cycloheximide at 10 .mu.g/ml for up to 260 min showed a reduction in ouabain-sensitive 86Rb+ uptake and total K+ content, with half-lives [t1/2] of 115 and 63 min, respectively. No consistent in vitro effect of prolactin on (Na+ + K+)-activated ATPase activity in homogenates, crude microsomal fractions or NaI-activated membrane fractions from lactating rabbit mammary gland was found. After 3 h of incubation of tissue slices in the presence or absence of prolactin (5 .mu.g/ml), no significant differences in the number of [G-3H]ouabain molecules bound per cell (5.2 .times. 104 and 6.2 .times. 104, respectively) or in Kd for ouabain binding (5.4 .times. 10-7 M and 5.9 .times. 10-7 M, respectively) were observed. Incubation of the tissue with cycloheximide (10 .mu.g/ml) for 1-6 h caused an exponential decrease in [G-3H]ouabain binding with a t1/2 of 3 h. Prolactin stimulates the activity of the (Na+ + K+)-activated ATPase in slices of lactating rabbit mammary gland within 1 h but over this period does not affect the number of ouabain-binding sites, which are apparently turned over with a t1/2 of 1-3 h.


Accession: 005264305



Related references

Effect of prolactin on 86Rb+ uptake, potassium content and [G-3H]ouabain binding of lactating rabbit mammary tissue. Journal of Physiology 334: 1-17, 1983

Effects of ouabain on tritium labeled ouabain binding rubidium 86 uptake cellular sodium and potassium and parathyroid hormone secretion in dispersed bovine parathyroid cells. Endocrinology 113(1): 371-378, 1983

Tritium labeled ouabain binding and effects on force of contraction and rubidium 86 uptake at different stimulation frequencies. Naunyn-Schmiedeberg's Archives of Pharmacology 319(SUPPL): R42, 1982

An effect of prolactin in potassium uptake by lactating rat mammary tissue. Hormone & Metabolic Research 23(6): 293-294, 1991

Tritium labeled ouabain binding and rubidium 86 uptake during the dimethyl sulfoxide induced differentiation of human promyelocytic leukemia hl 60 cells. Febs Letters 176(2): 467-472, 1984

Quantitation of tritium labeled ouabain binding and turnover of sodium potassium atpase along the rabbit nephron. American Journal of Physiology 247(1 PART 2): F158-F167, 1984

In vitro uptake and metabolism of tritium labeled cortico sterone by mammary glands from pregnant lactating and post lactational rats and by parametrial adipose tissue from lactating rats. Journal of Endocrinology 91(1): 81-88, 1981

Effect of potassium depletion on tritium labeled ouabain binding and sodium potassium contents in mammalian skeletal muscle. Acta Physiologica Scandinavica 122(2): 103-118, 1984

Tritium labeled ouabain binding and dissociation in rabbit colon effect of ions and drugs. Biochemical Pharmacology 28(21): 3181-3188, 1979

Uptake and binding of iodine 125 labeled prolactin by mammary tissue. Biochemical Journal: 8p-9p, 1972