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Effect of reductants on the spasmodic ph increase induced atp synthesis by membrane bound hydrogen ion atpase from lactobacillus casei

Malenkova, I.V.; Serezhenkov, V.A.; Davydov, R.M.; Blyumenfel'd L.A.

Biofizika 32(1): 164-166

1987


ISSN/ISBN: 0006-3029
Accession: 005266407

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It was shown that membrane bound ATPase from Lactobacillus casei synthetizes 5-10 nmol ATP/mg protein under pH jump from 6.0 to 8.0 in the presence of ascorbate only. ATP synthesis is carried out by nonequilibrium state of the enzyme with half-life time 5-7 s formed by ionization groups with pK = 6.8 - 7.0.

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