Effect of reduction and alkylation on the antigenicity of cartilage proteoglycans
Sugahara, K.; Dorfman, A.
Biochemical and Biophysical Research Communications 89(4): 1193-1199
ISSN/ISBN: 0006-291X PMID: 91369 DOI: 10.1016/0006-291x(79)92134-x
Immunological studies were performed on the proteoglycans from chick epiphyseal cartilage and rat chondrosarcoma. Immunodiffusion and direct radioimmune precipitation assays indicated that each proteoglycan subunit had species-specific and species-common antigenic determinants. Reduction and alkylation abolished the antigenicity of the species-specific antigenic determinants of both proteoglycan preparations as determined by immunodiffusion and radioimmune inhibition assays. Some, not all, of the species-common antigenic determinants also were sensitive to the treatment.