Effect of silicon di oxide liberated macrophage factor on protein synthesis in connective tissue in vitro

Aalto, M.; Turakainen, H.; Kulonen, E.

Scandinavian Journal of Clinical and Laboratory Investigation 39(3): 205-214


ISSN/ISBN: 0036-5513
Accession: 005269370

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To elucidate the role of macrophages in fibrosis the effect of the SiO2-liberated macrophage factor was assessed on protein synthesis in rat granulation-tissue slices. SiO2 was replaced by chrysotile asbestos. The active factor was found in the 44/55% sucrose interface of macrophage homogenate. There is no evidence of the involvement of collagenase. The SiO2 effect is not influenced by the addition of yeast RNA to the incubation medium. At a small concentration of polyvinylpyridine-N-oxide (PVNO) protein synthesis in the granulation tissue slices is stimulated, but at higher concentrations PVNO prevented the liberation of the fibrogenic factor from macrophages by SiO2. Phospholipase C and trypsin inhibited the effect of SiO2, which was partially abolished by heating, and repeated freezing and thawing. The macrophage RNAse, its inhibitors and fibroblast mRNA are suggested as key factors in the development of fibrosis.