Effect of storage of ethyl methanesulfonate and hydroxylamine treated seeds on mutation frequency in barley hordeum vulgare
Singh, R.M.; Agrawal, P.K.; Singh, J.; Singh, B.D.; Singh, R.B.
Indian Journal of Experimental Biology 16(10): 1067-1069
Seeds of Hordeum vulgare cv. IB-226 were treated with 0.03 M EMS [ethyl methanesulfonate] 0.08 M HA [hydroxylamine] or with a combination of the 2 mutagens for 6 h. The seeds were planted immediately or stored for 7 or 14 days. Immediate sowing resulted in lower biological damage in M1 [1st mutant genes stem], but higher mutation frequency and wider spectrum in M2. Storage reduced germination, seedling height and field survival to some extent, but pollen and ovule fertility were not appreciably affected. However, frequency and spectrum of chlorophyll and viable mutations were severely reduced by storage. Combination treatments showed a synergistic action in induction of chlorophyll and viable mutations.