Effect of the brachypod mutation on cell adhesion and chondrogenesis in aggregates of mouse limb mesenchyme

Duke, J.; Elmer, W.A.

Journal of Embryology and Experimental Morphology 42: 209-217


ISSN/ISBN: 0022-0752
Accession: 005276161

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A study of 12-day normal and brachypod mouse limb mesenchyme in rotation culture is described. Over a 3 1/2-h period, the rate of decline of single cells was significantly greater in mutant than in normal cultures, probably because the brachypod cells exhibited a greater degree of adhesiveness. The final size of the aggregates and their cell densities were the same by 24 h of incubation. Their pattern of chondrogenesis was different. Normal aggregates contained condensations with typical histotypic cartilage by 24 h of incubation, and were entirely chondrified by 4 days in culture. The condensations in brachypod aggregates were fewer, smaller, and delayed in their chondrogenesis. Never more than 50% of the brachypod aggregate exhibited chondrogenesis. The importance of cell contact and cell density to the chondrogenic process are discussed.