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Chapter 5,298

Effects of cordycepin on macromolecular synthesis and development in the preimplantation mouse embryo

Levey, I.L.; Brinster, R.L.

Experimental Cell Research 109(2): 397-405

1977


ISSN/ISBN: 0014-4827
PMID: 303178
Accession: 005297233

Investigations were conducted to test the effects of cordycepin, a naturally-occurring analog of adenosine, on gene activity in preimplantation mouse embryos. Embryos were explanted into culture at the 2-cell, morula and blastocyst stages and incubated in the absence or presence of cordycepin (5-100 .mu.g/ml) to determine the effects of the drug on continued development and macromolecular synthesis. Cordycepin at concentrations exceeding 10 .mu.g/ml caused a dose-responsive inhibition of cleavage and blastulation of embryos in culture. Exposure of morulae and blastocysts to cordycepin concentrations of 10-100 .mu.g/ml produced a dose- and time-dependent suppression of RNA synthesis as measured by incorporation of 3H-uridine. Suppression in blastocyst-stage embryos was enhanced by preincubation and reached 70% after 4 h at 100 .mu.g/ml. Cordycepin (50-100 .mu.g/ml) reduced synthesis of major RNA components detected by electrophoresis, blocked incorporation of radioactivity into fractions bound by olido(dT)-cellulose, and produced a time- and dose-dependent reduction of protein synthesis in blastocysts, causing a maximum inhibition of 25% after 4 h of preincubation at 50 .mu.g/ml.

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