Effects of extracorporeal membrane oxygenation on the values of lactic dehydrogenase glutamic oxal acetic trans aminase glutamic pyruvic trans aminase serum protein and free plasma hemo globin
Japanese Journal of Anesthesiology 29(13): 1617-1624
A Kolobow type membrane oxygenator was primed with lactated Ringer's solution or artificial blood (Fluosol-DA). An extracorporeal bypass circulation from the jugular vein into the carotid artery via the oxygenator was made by using a roller pump. Thus, the extracorporeal membrane oxygenation (ECMO) was performed for up to 7 days in 17 animals including 6 dogs, 6 sheep and 5 goats. Of 17 animals, 9 survived ECMO with normal and stable values of LDH [lactic dehydrogenase], GOT [glutamic oxalacetic transaminase] and GPT [glutamic pyruvic transaminase]. Animals with elevated values of these enzymes died during ECMO or soon after the end of ECMO. Serum protein decreased at the beginning of ECMO in relation to the degree of hemodilution with the priming fluid. In most cases it recovered gradually to the pre-bypass levels during or after ECMO. Exceptionally low serum protein values were seen only in animals with massive bleeding. Though an extreme hemolysis resulting in a high value of free plasma Hb was found in a goat when Fluosol-DA was used, the ECMO itself did not increase free plasma Hb in other animals. Any case that lead to unexpected death in the animal, also elevated the LDH, GOT and GPT values. The values of these enzymes are good indicators of the status of the experimental animals demonstrating a successful performance of ECMO.